Publication

2005

English

http://archimer.ifremer.fr/doc/2005/publication-365.pdf (0.17 Mo)

2004 Elsevier SAS All rights reserved

Postec Anne¹, Pignet Patricia¹, Cueff-Gauchard Valerie¹, Schmitt Anne¹, Querellou Joel¹, Godfroy Anne¹

1 : Ifremer, Ctr Brest, UMR 6197 , Lab Microbiol Environm Extremes, F-29280 Plouzane, France.

Research in Microbiology (0923-2508) (Elsevier), 2005 , Vol. 156 , N. 1 , P. 82-87

5

Biology

Bioreactor, Defined and minimal media, Culture optimisation, Gas lift bioreactor, Thermococcus hydrothermalis

The hyperthermophilic archaeon Thermococcus hydrothermalis was cultivated in continuous culture in a gas-lift bioreactor in the absence of elemental sulphur on both proteinaceous and maltose-containing media. Optimal conditions (pH, temperature and gas flow rate), determined on complex media that yielded maximal growth rate and maximal steady state cell density, were obtained at 80degreesC, pH 6 and gas sparging at 0.2 v v(-1) min(-1). Higher steady state cell densities were obtained on a medium containing maltose and yeast extract. In order to design a defined and minimal media, the nutritional requirements of T hydrothermalis were then investigated using continuous culture in the absence of elemental sulphur in the gas-lift bioreactor. First, the complex nutriments were replaced and a defined medium containing maltose, 19 amino acids and the two nitrogenous bases adenine and thymine, was determined. Secondly, selective feedings and withdrawal of amino acids showed requirements for 14 amino acids.