Publication

2005

English

http://archimer.ifremer.fr/doc/2005/publication-411.pdf (0.23 Mo)

EDP Sciences, IFREMER, IRD 2005

Peruzzi Stefano^{1, 2}, Chatain Beatrice¹, Menu Bruno¹

1 : IFREMER, Lab Rech Piscicole Mediterranee Chemin Maguelone, F-34250 Palavas Les Flots, France.
2 : Univ Tromso, Norwegian Coll Fishery Sci, N-9037 Tromso, Norway.

Aquatic Living Resources (0990-7440) (EDP Sciences), 2005 , Vol. 18 , N. 1 , P. 77-81

7

Biology

Anguilla anguilla, Liza ramada, Sparus aurata, Dicentrarchus labrax, Teleosts, DNA content

The nuclear DNA content in Dicentrarchus labrax, Sparus aurata, Liza ramada, and Anguilla anguilla, was measured by flow cytometric analysis. Male human leukocytes were used as internal reference cells. Nucleated fish erythrocytes were stained simultaneously to human leukocytes with a staining buffer containing propidium iodide (PI). Nuclear DNA contents of target species were estimated in relation to an assigned value of 7.0 pg DNA for male human leukocytes. The DNA content/nucleus (+/- CI) was 1.55 +/- 0.02 pg for D. labrax, 1.90 +/- 0.03 pg for S. aurata, 1.57 +/- 0.02 pg for L. ramada, and 2.43 +/- 0.04 pg for A. anguilla. Intraspecific DNA content variations ranged from 0-9% and averaged around 4% in all taxa. The results did not evidence significant differences in genome size between males and females in either D. labrax or L. ramada.