Shewanella profunda sp nov., isolated from deep marine sediment of the Nankai Trough
Type | Article | ||||||||
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Date | 2004-11 | ||||||||
Language | English | ||||||||
Author(s) | Toffin Laurent, Bidault Adeline, Pignet Patricia, Tindall Brian, Slobodkin Alexander, Kato Chiaki, Prieur Daniel | ||||||||
Affiliation(s) | CNRS, UMR 6539, F-29280 Plouzane, France. Univ Bretagne Occidentale, Technopole Brest Iroise, F-29280 Plouzane, France. Japan Marine Sci & Technol Ctr, Marine Ecosyst Res Dept, Yokosuka, Kanagawa 2370061, Japan. IFREMER, Ctr Brest, Dept Valorisat Prod, Lab Microbiol & Biotechnol Extremophiles, F-29280 Plouzane, France. Deutsch Sammlung Mikroorganismen & Zellkulturen, D-38124 Braunschweig, Germany. Russian Acad Sci, Inst Microbiol, Moscow 117312, Russia. |
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Source | International journal of systematic and evolutionary microbiology (1466-5026) (Society for General Microbiology), 2004-11 , Vol. 54 , N. 6 , P. 1943-1949 | ||||||||
DOI | 10.1099/ijs.0.03007-0 | ||||||||
WOS© Times Cited | 54 | ||||||||
Keyword(s) | Phylogeny, Bacteria, Microbiology, Deep sediment layer | ||||||||
Abstract | A novel piezotolerant, mesophilic, facultatively anaerobic, organotrophic, polarly flagellated bacterium (strain LT13a(T)) was isolated from a deep sediment layer in the Nankai Trough (Leg 190, Ocean Drilling Program) off the coast of Japan. This organism used a wide range of organic substrates as sole carbon and energy sources: pyruvate, glutamate, succinate, fumarate, lactate, citrate, peptone and tryptone. Oxygen, nitrate, fumarate, ferric iron and cystine were used as electron acceptors. Maximal growth rates were observed at a hydrostatic pressure of 10 MPa. Hydrostatic pressure for growth was in the range 0.1-50 MPa. Predominant cellular fatty acids were 16:1 omega7c, 15: 0 iso, 16: 0 and 13: 0 iso. The G + C content of the DNA was 44.9 mol%. On the basis of 16S rRNA gene sequences, strain LT13a(T) was shown to belong to the gamma-Proteobacteria, being closely related to Shewanella putrefaciens (98%), Shewanella oneidensis (97%) and Shewanella baltica (96 %). Levels of DNA homology between strain LT13a(T) and S. putrefaciens, S. oneidensis and S. baltica were < 20 %, indicating that strain LT13a(T) represents a novel species. Genetic evidence and phenotypic characteristics showed that isolate LT13a(T) constitutes a novel species of the genus Shewanella, Because of the deep origin of the strain, the name Shewanella profunda sp. nov. is proposed, with LT13a(T) (= DSM 15900(T)-JCM 12080(T)) as the type strain. | ||||||||
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