Detection of griseofulvin in a marine strain of Penicillium waksmanii by ion trap mass spectrometry
| Type | Article | ||||||||
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| Date | 2004-07 | ||||||||
| Language | English | ||||||||
| Author(s) | Petit Karina, Mondeguer Florence , Roquebert M, Biard J, Pouchus Y |
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| Affiliation(s) | Univ Nantes, Fac Pharm, ISOMer, SMAB, F-44035 Nantes 01, France. IFREMER, DEL, PN, F-44311 Nantes, France. Museum Natl Hist Nat, Lab Cryptogamie, F-75005 Paris, France. |
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| Source | Journal of Microbiological Methods (0167-7012) (Elsevier), 2004-07 , Vol. 58 , N. 1 , P. 59-65 | ||||||||
| DOI | 10.1016/j.mimet.2004.03.004 | ||||||||
| WOS© Times Cited | 23 | ||||||||
| Keyword(s) | Mass spectrometry ESI IT/MS, Griseofulvin, Antifungal, Marine fungi, Penicillium waksmanii | ||||||||
| Abstract | A marine strain of Penicillium waksmanii Zaleski was isolated from a sample of seawater from shellfish-farming area in the Loire estuary (France). The in vitro marine culture showed an important antifungal activity. Bioassay-guided fractionation was used to purify the crude extract. Dereplication by electrospray-ion trap/mass spectrometry (ESI-IT/MS) afforded the identification of the antifungal compound, after a semi-purification consisting of two stages. A comparison of the ionic composition between the active and the non-active fractions allowed the detection of a monocharged ion at m/z 353 containing a chlorine atom, which could be attributed to the antifungal griseofulvin [C17H17ClO6+H](+). Multi-stage fragmentation (MSn) confirmed the identity of the m/z 353 ion of the antifungal fraction as griseofulvin. It is the first description of griseofulvin production by a strain of P. waksmanii and the first chemical study of a strain of this species isolated from marine temperate cold water. | ||||||||
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