Publication

2005-10

English

http://archimer.ifremer.fr/doc/2005/publication-679.pdf (0.37 Mo)

2005 Elsevier Inc. All rights reserved

Zemani Faouzia¹, Benisvy Danielle¹, Galy Fauroux Isabelle¹, Lokajczyk Anna¹, Colliec-Jouault Sylvia², Uzan Georges³, Fischer Anne Marie¹, Boisson Vidal Catherine¹

1 : INSERM, Fac Pharm, U428, F-75270 Paris, France.
2 : IFREMER, Nantes, France.
3 : Hop Paul Brousse, INSERM, U506, Villejuif, France.

Biochemical Pharmacology (0006-2952) (Elsevier), 2005-10 , Vol. 70 , N. 8 , P. 1167-1175

17

Biology

Fucoidan, FGF 2, Endothelial progenitor cells, Angiogenesis, Alpha 6

Endothelial progenitor cell (EPC) transplantation is a potential means of inducing neovascularization in vivo. However, the number of circulating EPC is relatively small, it may thus be necessary to enhance their proangiogenic properties ex vivo prior to injection in vivo. Fucoidan has previously been shown to potentiate in vitro tube formation by mature endothelial cells in the presence of basic fibroblast growth factor (FGF-2). We therefore examined whether fucoidan, alone or combined with FGF-2, could increase EPC proangiogenic potency in vitro. EPC exposure to 10 mu g/ml fucoidan induced a proangiogenic phenotype, including cell proliferation (p < 0.01) and migration (p < 0.01); moreover, differentiation into vascular cords occurred in the presence of FGF-2 (p < 0.01). This latter effect correlated with upregulation of the cell-surface alpha 6 integrin subunit of the laminin receptor (P < 0.05). Compared to untreated HUVEC, untreated EPC alpha 6 expression and adhesion to laminin were enhanced two-fold. Fucoidan treatment further enhanced HUVEC but not EPC adhesion to laminin. These results show that fucoidan enhances the proangiogenic properties of EPC and suggest that ex vivo cc fucoidan preconditioning of EPC might lead to increased neovascularization when injected into ischemic tissues.