Gas chromatographic behavior of fatty acid derivatives for mass spectrometry on low-polarity capillary columns

Type Article
Date 2009-07
Language English
Author(s) Dubois Nolwenn, Barnathan Gilles2, Gouygou Jean-Paul, Berge Jean-PascalORCID1
Affiliation(s) 1 : IFREMER, Ctr Nantes, Dept Sci & Tech Alimentaires Marines, Lab Sci & Tech Alimentaires Marines, F-44311 Nantes 3, France.
2 : Univ Nantes, Nantes Atlantique Univ, Fac Pharm, Grp Mer Mol Sante MMS,EA 2160, F-44035 Nantes, France.
Source European Journal of Lipid Science and Technology (1438-7697) (Wiley / Blackwell), 2009-07 , Vol. 111 , N. 7 , P. 688-697
DOI 10.1002/ejlt.200800148
WOS© Times Cited 11
Keyword(s) Picolinyl esters, N Acyl pyrrolidides, Methyl esters, GC MS, Fatty acid derivatives, 4,4 Dimethyloxazoline
Abstract The gas chromatographic properties of four derivatives of fatty acids (FA), namely fatty acid methyl esters, picolinyl esters, N-acyl pyrrolidides and 4,4-dimethyloxazoline derivatives, which contain various structural features (double bonds, branching, hydroxyl group) in their acyl chains have been compared on a low-polarity capillary column with a mass spectrometer as detector. Temperature programming rates yielding the highest resolution were optimized for each derivative by means of computer-assisted column temperature optimization software. Indeed, the Drylab software represents a valuable assistance for estimating the optimum analysis conditions. Time and efforts required for such method development can greatly be reduced. Different parameters (derivatization procedure, total run time, resolution and response factor) are discussed. N-Acyl pyrrolidides and picolinyl esters appear quite powerful for structure elucidation of polyunsaturated FA by GC-MS, and both these types of FA derivatives can be very well separated on a low-polarity phase.
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Dubois Nolwenn, Barnathan Gilles, Gouygou Jean-Paul, Berge Jean-Pascal (2009). Gas chromatographic behavior of fatty acid derivatives for mass spectrometry on low-polarity capillary columns. European Journal of Lipid Science and Technology, 111(7), 688-697. Publisher's official version : https://doi.org/10.1002/ejlt.200800148 , Open Access version : https://archimer.ifremer.fr/doc/00000/6846/