Stylicins, a new family of antimicrobial peptides from the Pacific blue shrimp Litopenaeus stylirostris
| Type | Article | ||||||||||||
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| Date | 2010-03 | ||||||||||||
| Language | English | ||||||||||||
| Author(s) | Rolland Jean-Luc4, Abdelouahab Mahdia2, Dupont J.3, Lefevre F.3, Bachere Evelyne4, Romestand Bernard1 | ||||||||||||
| Affiliation(s) | 1 : Univ Montpellier 2, IFREMER, CNRS, IRD,UMR Ecosyst Lagunaires 5119, F-34095 Montpellier 5, France. 2 : Catholic Univ Louvain, Unite Microbiol MBLA, B-1348 Louvain, Belgium. 3 : LibraGen, F-31400 Toulouse, France. 4 : IFREMER, Univ Montpellier 2, CNRS, IRD,UMR Ecosyst Lagunaires 5119, F-34095 Montpellier 5, France. |
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| Source | Molecular Immunology (0161-5890) (Pergamon-elsevier Science Ltd), 2010-03 , Vol. 47 , N. 6 , P. 1269-1277 | ||||||||||||
| DOI | 10.1016/j.molimm.2009.12.007 | ||||||||||||
| WOS© Times Cited | 48 | ||||||||||||
| Keyword(s) | Ls-Stylicin 1, Peptide, Shrimp, Antifungal, Agglutination, Vibrio | ||||||||||||
| Abstract | The present study reports the characterization of Ls-Stylicin1, a novel antimicrobial peptide from the penaeid shrimp, Litopenoeus stylirostris. The predicted mature peptide of 82 residues is negatively charged (theoretical pl=5.0) and characterized by a proline-rich N-terminal region and a C-terminal region containing 13 cysteine residues. The recombinant Ls-Stylicin1 has been isolated in both monomeric and dimeric forms. Both display strong antifungal activity against Fusarium oxysporum (1.25 mu M < MIC <2.5 mu M), a pathogenic fungus of shrimp, but lower antimicrobial activity against Gram () bacteria, Vibrio sp. (40 mu M < MIC <80 mu M). However, rLs-Stylicin1 is able to agglutinate Vibrio pennaeicidae in vitro in agreement with its potent LPS-binding activity on immobilized LPS of V. penaeicidae (dissociation constant (K-d) of 9.6 x 10(-8) M). This molecule with no evident homology to other hitherto described antimicrobial peptides but identified herein several species of penaeid shrimp is thought to be the first member of a shrimp antimicrobial peptide family, which we termed stylicins. (C) 2009 Elsevier Ltd. All rights reserved. | ||||||||||||
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