The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas
| Type | Article | ||||||||||||
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| Date | 2010-04 | ||||||||||||
| Language | English | ||||||||||||
| Author(s) | Duperthuy Marylise1, 2, Binesse Johan3, Le Roux Frederique3, 4, Romestand Bernard1, 2, Caro Audrey1, 2, Got Patrice1, 2, Givaudan Alain5, 6, Mazel Didier3, Bachere Evelyne1, 2, Destoumieux Garzon Delphine1, 2 | ||||||||||||
| Affiliation(s) | 1 : Univ Montpellier 2, CNRS, UMR 5119, Lab Ecosyst Iagunaires, F-34095 Montpellier, France. 2 : IFREMER, F-34095 Montpellier, France. 3 : Inst Pasteur, URA 2171, CNRS, Lab Plast Genome Bacterien, F-75015 Paris, France. 4 : IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France. 5 : Univ Montpellier 2, Lab Ecol Microbienne Insectes & Interact Hote Pat, F-34095 Montpellier, France. 6 : INRA, F-34095 Montpellier, France. |
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| Source | Environmental Microbiology (1462-2912) (Wiley-blackwell Publishing, Inc), 2010-04 , Vol. 12 , N. 4 , P. 951-963 | ||||||||||||
| DOI | 10.1111/j.1462-2920.2009.02138.x | ||||||||||||
| WOS© Times Cited | 75 | ||||||||||||
| Abstract | Vibrio splendidus, strain LGP32, is an oyster pathogen associated with the summer mortalities affecting the production of Crassostrea gigas oysters worldwide. Vibrio splendidus LGP32 was shown to resist to up to 10 mu M Cg-Def defensin and Cg-BPI bactericidal permeability increasing protein, two antimicrobial peptides/proteins (AMPs) involved in C. gigas immunity. The resistance to both oyster Cg-Def and Cg-BPI and standard AMPs (polymyxin B, protegrin, human BPI) was dependent on the ompU gene. Indeed, upon ompU inactivation, minimal bactericidal concentrations decreased by up to fourfold. AMP resistance was restored upon ectopic expression of ompU. The susceptibility of bacterial membranes to AMP-induced damages was independent of the ompU-mediated AMP resistance. Besides its role in AMP resistance, ompU proved to be essential for the adherence of V. splendidus LGP32 to fibronectin. Interestingly, in vivo, ompU was identified as a major determinant of V. spiendidus pathogenicity in oyster experimental infections. Indeed, the V. splendidus-induced oyster mortalities dropped from 56% to 11% upon ompU mutation (Kaplan Meier survival curves, P < 0.01). Moreover, in co-infection assays, the ompU mutant was out competed by the wild-type strain with competitive indexes in the range of 0.1-0.2. From this study, ompU is required for virulence of V. splendidus. Contributing to AMP resistance, conferring adhesive properties to V. splendidus, and being essential for in vivo fitness, the OmpU porin appears as an essential effector of the C. gigas/V. splendidus interaction. | ||||||||||||
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