FN Archimer Export Format
PT J
TI PCR-RFLP analyses of formalin-fixed fish eggs for the mapping of spawning areas in the Eastern Channel and Southern North Sea
BT 
AF LELIEVRE, Stephanie
   VERREZ-BAGNIS, Veronique
   JEROME, Marc
   VAZ, Sandrine
AS 1:1;2:2;3:2;4:1;
FF 1:PDG-DOP-DCMMN-HMMN-RHBL;2:PDG-DOP-DCN-BRM-STBM;3:PDG-DOP-DCN-BRM-STBM;4:PDG-DOP-DCMMN-HMMN-RHBL;
C1 IFREMER, Dept Halieut Resources, Boulogne, France.
   IFREMER, Dept Biotechnol & Marine Resources BRM, Nantes, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
SI BOULOGNE
   NANTES
SE PDG-DOP-DCMMN-HMMN-RHBL
   PDG-DOP-DCN-BRM-STBM
IN WOS Ifremer jusqu'en 2018
IF 1.749
TC 15
UR https://archimer.ifremer.fr/doc/00005/11613/8259.pdf
LA English
DT Article
CR CGFS2008
   CGFS2009
   CGFS2010
   IBTS 2008
BO Gwen Drez
   Thalassa
DE ;fish eggs;PCR-RFLP;cytochrome b gene;geostatistical analyses;interpolated map
AB A better knowledge on spawning grounds of principal commercial fishes from North Sea is necessary for their monitoring. Fish egg taxonomic identification based on morphological characters cannot always be carried out effectively. This is particularly the case for a number of Gadidae fish species eggs (i.e. cod, haddock and whiting, and three rockling species) which have the same range of size and for which molecular-based techniques can help improving the accuracy of egg taxonomic identification, and hence the reliability of studies on spawning areas. In this study, formalin fixation, egg conservation and DNA extraction were optimized in order to develop a specific PCR-RFLP method. Based on the sequence of the cytochrome b gene of reference specimens, a PCR-RFLP method was developed to distinguish eggs of cod, haddock and whiting of one part, and between eggs from three species of rocklings on the other part. Among the 404 eggs tested, 80% of positive amplification was obtained for rocklings eggs and 86.36 % for Gadidae eggs. Eggs from Enchelyopus cimbrius, Ciliata mustela and Ciliata septentrionalis, and Gadus morhua, Merlangius merlangus and Melanogrammus aeglefinus were distinguished. with this method. Based on morphological characters, the percentage of misidentification was less for cod than whiting.
PY 2010
PD NOV
SO Journal Of Plankton Research
SN 0142-7873
PU Oxford Univ Press
VL 32
IS 11
UT 000282750800004
BP 1527
EP 1539
DI 10.1093/plankt/fbq067
ID 11613
ER

EF