Effect of diets containing different levels of highly unsaturated fatty acids on physiological and immune responses in Pacific whiteleg shrimp Litopenaeus vannamei (Boone) exposed to handling stress
| Type | Article | ||||||||||||
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| Date | 2009-11 | ||||||||||||
| Language | English | ||||||||||||
| Author(s) | Mercier Laurence1, Racotta Ilie, Yepiz-Plascencia Gloria2, Muhlia-Almazan Adriana2, Civera Roberto1, Quinones-Arreola Marcos F.1, Wille Mathieu3, 4, Sorgeloos Patrick3, 4, Palacios Elena | ||||||||||||
| Affiliation(s) | 1 : Ctr Invest Biol Noroeste, La Paz 23090, Baja California, Mexico. 2 : Ctr Invest Alimentac & Desarrollo, Hermosillo, Sonora, Mexico. 3 : Univ Ghent, Fac Biosci Engn, Lab Aquaculture, B-9000 Ghent, Belgium. 4 : Univ Ghent, Fac Biosci Engn, Artemia Reference Ctr, B-9000 Ghent, Belgium. |
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| Source | Aquaculture Research (1355-557X) (Wiley-blackwell Publishing, Inc), 2009-11 , Vol. 40 , N. 16 , P. 1849-1863 | ||||||||||||
| DOI | 10.1111/j.1365-2109.2009.02291.x | ||||||||||||
| WOS© Times Cited | 39 | ||||||||||||
| Keyword(s) | anion superoxide, clotting time, glucose, haemocyte, HDL-BGBP, HUFA | ||||||||||||
| Abstract | Juveniles fed a diet containing a low or a high level of highly unsaturated fatty acids (HUFA) for 38 days were exposed to handling stress. In a first experiment, stress was applied daily for 30 days, after which the physiological and immunological variables were measured, whereas in a second experiment, stress was applied once and samples were obtained 1 and 24 h after the stressor event. Shrimp that were stressed for 30 days showed significantly lower survival, final weight and feed consumption compared with unstressed shrimp. The concentration of the high-density lipoprotein beta-glucan-binding protein was significantly higher in shrimp fed the high-HUFA diet. The glucose concentration in the haemolymph was significantly higher in long-term stressed shrimp compared to controls. The lactate level in the haemolymph was significantly lower in shrimp fed the high-HUFA diet. Lactate and glucose in the haemolymph increased in the 1-h stressed shrimp, but returned to normal levels in 24-h stressed shrimp. A negative effect of repeated-handling stress applied for 30 days was mainly observed on biological performance, whereas the single-stressor event had a more pronounced effect on shrimp physiological and immune responses measured 1 and 24 h after the stressor. A beneficial role of enrichment with HUFA on tolerance to handling stress was observed on immune response capacity. | ||||||||||||
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