A mussel tissue certified reference material for multiple phycotoxins. Part 1: design and preparation
Type | Article | ||||||||||||
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Date | 2011-05 | ||||||||||||
Language | English | ||||||||||||
Author(s) | McCarron Pearse1, 2, Emteborg Hakan3, Nulty Ciara2, Rundberget Thomas4, Loader Jared I.5, Teipel Katharina3, Miles Christopher O.4, 5, Quilliam Michael A.1, Hess Philipp2 | ||||||||||||
Affiliation(s) | 1 : Natl Res Council Canada, Inst Marine Biosci, Halifax, NS B3H 3Z1, Canada. 2 : Inst Marine, Galway, Ireland. 3 : Commiss European Communities, Joint Res Ctr, Inst Reference Mat & Measurements, B-2440 Geel, Belgium. 4 : Natl Vet Inst, N-0106 Oslo, Norway. 5 : AgResearch Ltd, Hamilton 3240, New Zealand. |
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Source | Analytical And Bioanalytical Chemistry (1618-2642) (Springer Heidelberg), 2011-05 , Vol. 400 , N. 3 , P. 821-833 | ||||||||||||
DOI | 10.1007/s00216-011-4786-9 | ||||||||||||
WOS© Times Cited | 6 | ||||||||||||
Keyword(s) | CRM-FDMT1, Certified reference material, Shellfish toxins, Phycotoxins, Accuracy, Precision, Liquid chromatography-mass spectrometry | ||||||||||||
Abstract | The development of multi-analyte methods for lipophilic shellfish toxins based on liquid chromatography-mass spectrometry permits rapid screening and analysis of samples for a wide variety of toxins in a single run. Validated methods and appropriate certified reference materials (CRMs) are required to ensure accuracy of results. CRMs are essential for accurate instrument calibration, for assessing the complete analytical method from sample extraction to data analysis and for verifying trueness. However, CRMs have hitherto only been available for single toxin groups. Production of a CRM containing six major toxin groups was achieved through an international collaboration. Preparation of this material, CRM-FDMT1, drew on information from earlier studies as well as improved methods for isolation of toxins, handling bulk tissues and production of reference materials. Previous investigations of stabilisation techniques indicated freeze-drying to be a suitable procedure for preparation of shellfish toxin reference materials and applicable to a wide range of toxins. CRM-FDMT1 was initially prepared as a bulk wet tissue homogenate containing domoic acid, okadaic acid, dinophysistoxins, azaspiracids, pectenotoxin-2, yessotoxin and 13-desmethylspirolide C. The homogenate was then freeze-dried, milled and bottled in aliquots suitable for distribution and analysis. The moisture content and particle size distribution were measured, and determined to be appropriate. A preliminary toxin analysis of the final material showed a comprehensive toxin profile. | ||||||||||||
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