The influence of dietary supplementation of arachidonic acid on prostaglandin production and oxidative stress in the Pacific oyster Crassostrea gigas
| Type | Article | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Date | 2011-09 | ||||||||||||
| Language | English | ||||||||||||
| Author(s) | Seguineau Catherine1, Racotta Ilie2, 3, Palacios Elena3, 4, Delaporte Maryse2, Moal Jeanne2, Soudant Philippe4 | ||||||||||||
| Affiliation(s) | 1 : Université de Bretagne Occidentale, Brest, France et UMR 100 Physiologie et Ecophysiologie des Mollusques Marins, Centre IFREMER de Brest, BP70, 29280 Plouzané, France 2 : UMR 100 Physiologie et Ecophysiologie des Mollusques Marins, Centre IFREMER de Brest, BP70, 29280 Plouzané, France 3 : Programa de Acuicultura, Centro de Investigaciones Biológicas del Noroeste, Apdo. Postal 128, La Paz B.C.S. 23090, Mexico 4 : Laboratoire des Sciences de l'Environnement Marin, UMR 6539, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, Technopôle Brest-Iroise, 29280 Plouzané, France |
||||||||||||
| Source | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology (1095-6433) (Elsevier), 2011-09 , Vol. 160 , N. 1 , P. 87-93 | ||||||||||||
| DOI | 10.1016/j.cbpa.2011.05.011 | ||||||||||||
| WOS© Times Cited | 8 | ||||||||||||
| Keyword(s) | Prostaglandins, n-6 unsaturated fatty acids, Lipid peroxidation, Antioxidants, ROS production | ||||||||||||
| Abstract | In a previous study, dietary supplementation with arachidonic acid (ARA) to oysters Crassostrea gigas increased haemocyte numbers, phagocytosis, and production of reactive oxygen species level (ROS) by haemocytes (Delaporte et al., 2006). To assess if the observed stimulation of these cellular responses resulted from changes of ARA-related prostaglandin (PG) production, we analysed prostaglandin E2 metabolite (PGEM) content on the same oysters fed three levels of ARA. Dietary supply of polyunsaturated fatty acids (PUFA) could also induce an oxidative stress that could similarly increase cellular responses; therefore, two indicators of oxidative stress were analysed: peroxidation level and antioxidant defence status. Together the observed positive correlation between ARA and PGEM levels and the absence of lipid peroxidation and antioxidant activity changes supports the hypothesis of an immune stimulation via PG synthesis. Although ARA proportion in oyster tissues increased by up to 7-fold in response to ARA dietary supplementation, peroxidation index did not change because of a compensatory decrease in n-3 fatty acid proportion, mainly 22:6n-3. To further confirm the involvement of PG in the changes of haemocyte count, phagocytosis and ROS production upon ARA supplementation, it would be interesting to test cyclooxygenase and lipooxygenase inhibitors in similar experiments. | ||||||||||||
| Full Text |
|