Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" - First Periodic Progress Report 4th January 1999 to 3rd January 2000

Type Contract report
Date 2000
Language English
Ref. Contract FAIR-CT98-4334 - European Commission
Author(s) Renault Tristan, Davison Andrew, Xhonneux Florence, Dorange Germaine, Culloty Sarah, Novoa Beatriz, Dixon Peter
Keyword(s) Pathology, Diagnosis, Oyster, Herpes like virus, Herpesviridae, Immunology, Detection, Virus, Bivalves
Abstract The aim of the programme is to develop specific tools for diagnosing herpes-like virus infections in bivalves and to validate these reagents by using them in different European laboratories involved in shellfish epidemiological surveys. This will done using techniques developed by the partners to characterise viruses by studying their genomes and immunologically reactive proteins, to cultivate oyster cells and vertebrate cell lines and to perform epidemiological surveys among bivalves. The programme objectives are: 1 - Obtaining the complete oyster herpes-like virus DNA sequence with determination of the genome structure. 2 - Comparing oyster herpes-like viruses with other viruses belonging to the Helpesviridae family on the basis of sequence data and genome structure. 3 - Developing molecular tools for oyster herpes-like virus detection using the complete virus DNA sequence data. 4 - Developing immunological tools for oyster herpes-like virus detection. 5 - Developing cellular tools for oyster herpes-like virus detection using oyster primary cell cultures and vertebrate cell lines. 6 - Application of developed diagnostic tools for herpes-like virus detection in oyster samples from different geographical locations.
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Renault Tristan, Davison Andrew, Xhonneux Florence, Dorange Germaine, Culloty Sarah, Novoa Beatriz, Dixon Peter (2000). Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" - First Periodic Progress Report 4th January 1999 to 3rd January 2000. Contract FAIR-CT98-4334 - European Commission. https://archimer.ifremer.fr/doc/00044/15523/