FN Archimer Export Format PT J TI Mesophilic sulfate-reducing bacteria from 3 deep-sea hydrothermal vent sites BT AF ELSGAARD, L GUEZENNEC, J BENBOUZIDROLLET, N PRIEUR, D AS 1:;2:;3:;4:; FF 1:;2:;3:;4:; C1 CNRS,BIOL STN,F-29682 ROSCOFF,FRANCE IFREMER,CTR BREST,DRO EP,BIOTECHNOL MICROORGANISMES HYDROTHERMAUX LAB,F-29280 PLOUZANE,FRANCE UNIV PARIS 06,F-29682 ROSCOFF,FRANCE C2 CNRS,BIOL STN,F-29682 ROSCOFF,FRANCE IFREMER,CTR BREST,DRO EP,BIOTECHNOL MICROORGANISMES HYDROTHERMAUX LAB,F-29280 PLOUZANE,FRANCE UNIV PARIS 06,F-29682 ROSCOFF,FRANCE IF 0.662 TC 15 UR https://archimer.ifremer.fr/doc/00097/20861/18479.pdf LA English DT Article AB The present study was conducted to determine the presence and physiology of mesophilic sulfate-reducing bacteria (SRB) at deep-sea hydrothermal vents, which constitute remote ecosystems, largely dependent on their own chemoautotrophic primary production. SRB were enriched and isolated from samples of hydrothermal water, invertebrates, chimneys, and sediment collected at deep-sea (1700 to 2600 m) hydrothermal vent sites in the Lau Basin, in the North Fiji Basin, and at 13 degrees N on the East Pacific Rise. From the hydrothermal fields in the Lau Basin and the North Fiji Basin, SRB were cultured at 30 degrees C from 19 out of 21 samples, including five samples of high-temperature hydrothermal water (> 100 degrees C). Acetate, benzoate, formate, isobutyrate, and lactate were supplied as single electron donors and could all be degraded by SRB. From the site at 13 degrees N, SRB were enriched at 20 or 40 degrees C from seven out of 20 samples. Two Desulfovibrio. strains, H 2.5 and H 5.3, originated from the tubes of the polychaete Alvinella sp. and these strains had optimum temperatures at 37 to 40 degrees C with doubling times of 3.6 and 6.6 hours, respectively. In cultures of strain H 2.5, which had reached the stationary growth phase, almost the entire populations (> 99%) changed into round pleomorphs (coccoid bodies). However, cultures of vibrio-shaped bacteria were recovered upon transfer of an inoculum to fresh medium. No distinct changes in the composition of the cell membrane phospholipid fatty acids were associated with the morphological transformation. In the deep-sea hydrothermal vent environment, mesophilic SRB occurred frequently and may thrive in anoxic habitats in association with warm vent animal communities. PY 1995 SO Oceanologica Acta SN 0399-1784 PU Gauthier-Villars VL 18 IS 1 UT A1995RZ83200007 BP 95 EP 104 ID 20861 ER EF