TY - JOUR T1 - Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas A1 - Corporeau,Charlotte A1 - Vanderplancke,Gwenaelle A1 - Boulais,Myrina A1 - Suquet,Marc A1 - Quere,Claudie A1 - Boudry,Pierre A1 - Huvet,Arnaud A1 - Madec,Stephanie AD - IFREMER, Lab Sci Environm Marin, UMR 6539, LEMAR, F-29280 Plouzane, France. AD - Univ Europeenne Bretagne, ESMISAB, SdnBioS IFR148, Lab Univ Biodivers & Ecol Microbienne EA3882, F-29280 Plouzane, France. UR - https://doi.org/10.1016/j.jprot.2012.07.040 DO - 10.1016/j.jprot.2012.07.040 KW - Marine bivalves KW - Crassostrea gigas KW - Oocyte quality KW - Proteomics N2 - We used a 2-DE proteomic approach to identify abundant proteins linked to oocyte quality in the Pacific oyster Crassostrea gigas, an economically important bivalve. Oocyte quality of 14 females was estimated by recording fertilisation and early developmental success until D-larval stage under controlled conditions. Proteins that were differentially expressed between females showing high or low oocyte quality were identified by nano-liquid chromatography tandem mass spectrometry. Twelve up-accumulated spots associated with low quality oocytes revealed 10 distinct proteins, including vitellogenin — breakdown products and metabolic enzymes. Eight up-accumulated spots from high quality oocytes revealed 6 distinct proteins, including chaperone molecules and cell-cycle control proteins. This is the first proteomic study dedicated to oocytes in C. gigas. Our results improve current knowledge about protein factors associated with oocyte quality in this species, and our understanding of the proteomic processes involved in their developmental competence. Highlights ► 14 Pacific oyster females were fertilised in vitro by a common pool of spermatozoa. ► Individual variability of D-larva yields was due to the intrinsic quality of oocytes. ► Two-dimensional proteomics identified protein factors for oocyte quality. Y1 - 2012/10 PB - Elsevier Science Bv JF - Journal Of Proteomics SN - 1874-3919 VL - 75 IS - 18 SP - 5554 EP - 5563 ID - 20925 ER -