FN Archimer Export Format PT J TI Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas BT AF CORPOREAU, Charlotte VANDERPLANCKE, Gwenaelle BOULAIS, Myrina SUQUET, Marc QUERE, Claudie BOUDRY, Pierre HUVET, Arnaud MADEC, Stephanie AS 1:1;2:1;3:1;4:1;5:1;6:1;7:1;8:2; FF 1:PDG-RBE-PFOM-PI;2:PDG-RBE-PFOM-ARN;3:PDG-RBE-PFOM-PI;4:PDG-RBE-PFOM-PI;5:PDG-RBE-PFOM-PI;6:PDG-RBE-PFOM-PI;7:PDG-RBE-PFOM-PI;8:; C1 IFREMER, Lab Sci Environm Marin, UMR 6539, LEMAR, F-29280 Plouzane, France. Univ Europeenne Bretagne, ESMISAB, SdnBioS IFR148, Lab Univ Biodivers & Ecol Microbienne EA3882, F-29280 Plouzane, France. C2 IFREMER, FRANCE ESMISAB, FRANCE SI BREST ARGENTON SE PDG-RBE-PFOM-PI PDG-RBE-PFOM-ARN IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 4.09 TC 45 UR https://archimer.ifremer.fr/doc/00098/20925/18534.pdf LA English DT Article DE ;Marine bivalves;Crassostrea gigas;Oocyte quality;Proteomics AB We used a 2-DE proteomic approach to identify abundant proteins linked to oocyte quality in the Pacific oyster Crassostrea gigas, an economically important bivalve. Oocyte quality of 14 females was estimated by recording fertilisation and early developmental success until D-larval stage under controlled conditions. Proteins that were differentially expressed between females showing high or low oocyte quality were identified by nano-liquid chromatography tandem mass spectrometry. Twelve up-accumulated spots associated with low quality oocytes revealed 10 distinct proteins, including vitellogenin — breakdown products and metabolic enzymes. Eight up-accumulated spots from high quality oocytes revealed 6 distinct proteins, including chaperone molecules and cell-cycle control proteins. This is the first proteomic study dedicated to oocytes in C. gigas. Our results improve current knowledge about protein factors associated with oocyte quality in this species, and our understanding of the proteomic processes involved in their developmental competence. Highlights ► 14 Pacific oyster females were fertilised in vitro by a common pool of spermatozoa. ► Individual variability of D-larva yields was due to the intrinsic quality of oocytes. ► Two-dimensional proteomics identified protein factors for oocyte quality. PY 2012 PD OCT SO Journal Of Proteomics SN 1874-3919 PU Elsevier Science Bv VL 75 IS 18 UT 000311196600004 BP 5554 EP 5563 DI 10.1016/j.jprot.2012.07.040 ID 20925 ER EF