DNA hybridization mechanism in an interfacial environment: What hides beneath first order k (s(-1)) kinetic constant?
| Type | Article | ||||||||||||
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| Date | 2012-08 | ||||||||||||
| Language | English | ||||||||||||
| Author(s) | Lazerges M.1, 2, Perrot H.1, 2, Rabehagasoa N.3, Compere Chantal 3, Dreanno Catherine3, Mucio Pedroso M.4, Faria R. C.4, Bueno P. R.5 |
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| Affiliation(s) | 1 : CNRS, UPR 15, Lab Interfaces & Syst Electrochim, F-75252 Paris, France. 2 : Univ Paris 06, LISE, F-75252 Paris, France. 3 : IFREMER, Ctr Brest, Serv Interfaces & Capteurs, ZI Pointe Diable, F-29280 Plouzane, France. 4 : Univ Fed Sao Carlos, Dept Quim, BR-13560905 Sao Paulo, Brazil. 5 : Univ Estadual Paulista, Dept Fisicoquim, Inst Quim, BR-14800900 Sao Paulo, Brazil. |
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| Source | Sensors And Actuators B-chemical (0925-4005) (Elsevier Science Sa), 2012-08 , Vol. 171 , P. 522-527 | ||||||||||||
| DOI | 10.1016/j.snb.2012.05.023 | ||||||||||||
| WOS© Times Cited | 5 | ||||||||||||
| Keyword(s) | Biosensors, DNA, Hybridization kinetics, Quartz crystal microbalance | ||||||||||||
| Abstract | The scientific question addressed in this work is: what hides beneath first order kinetic constant k (s(-1)) measured for hybridization of a DNA target on a biosensor surface. Kinetics hybridization curves were established with a 27 MHz quartz microbalance (9 MHz, third harmonic) biosensor, constituted of a 20-base probe monolayer deposited on a gold covered quartz surface. Kinetics analysis, by a known two-step adsorption-hybridization mechanism, is well appropriate to fit properly hybridization kinetics curves, for complementary 20-base to 40-base targets over two concentration decades. It was found that the K-1 (M-1) adsorption constant, relevant to the first step, concerns an equilibrium between non hybridized targets and hybridized pre-complex and increases with DNA target length. It was established that k(2) (s(-1)), relevant to irreversible formation of a stable duplex, varies in an opposite way to K-1 with DNA target length. (C) 2012 Published by Elsevier B.V. | ||||||||||||
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