Short Technical Report for OsHV-1 detection and quantification by Real Time Polynerase Chain Reaction using OsHV-1 DNA polymerase sequence

Type Report
Date 2013-04
Language English
Ref. RST/LER-PC/13-04
Author(s) Pepin Jean-Francois1
Affiliation(s) 1 : Ifremer, Laboratoire Environnement Ressource des Pertuis charentais, Ifremer, La Tremblade, France
Mot-Clé(s) PCR quantitative HDP-F/R, virus OsHV-1, diagnostic, DNA polymerase, validation
Keyword(s) Real-Time PCR HDP-F/R, OsHV-1, Diagnostic, DNA polymerase, Validation
Abstract The present report completes a previously described procedure routinely used for the detection and quantification of OsHV-1 by Sybr green Real Time Polymerase Chain Reaction targeting the OsHV-1 DNA polymerase sequence. More particularly, this document brings information about analytical sensitivity, quantitation limit, assay precision, reproducibility of the technique.
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Pepin Jean-Francois (2013). Short Technical Report for OsHV-1 detection and quantification by Real Time Polynerase Chain Reaction using OsHV-1 DNA polymerase sequence. RST/LER-PC/13-04. https://archimer.ifremer.fr/doc/00137/24814/