Type |
Article |
Date |
2014-05 |
Language |
English |
Author(s) |
Vincent-Hubert Francoise1, Chatel Amelie1, Gourlay-France Catherine1 |
Affiliation(s) |
1 : IRSTEA, Unite Rech Hydrosyst & Bioprocedes, F-92761 Antony, France. |
Source |
Mutation Research-genetic Toxicology And Environmental Mutagenesis (1383-5718) (Elsevier Science Bv), 2014-05 , Vol. 766 , P. 10-15 |
DOI |
10.1016/j.mrgentox.2014.03.006 |
WOS© Times Cited |
6 |
Keyword(s) |
Benzo[a]pyrene, Cadmium, Metallothionein, Aryl hydrocarbon receptor, Antioxidant enzymes, RT-qPCR |
Abstract |
We have previously shown that cadmium (Cd) and Benzo[a]pyrene (BaP) induced early DNA damages in zebra mussels, and that the level of DNA strand breaks (SB) returned to a basal level after 3 days of exposure to Cd. The aim of the present study was to go further in the mechanisms of Cd and BaP detoxification. For that purpose, expression of genes encoding for metallothionein (MT), Aryl Hydrocarbon Receptor (AHR), P-gP, catalase, glutathione S-transferase and Heat shock protein 70 (HSP70) proteins have been measured using RT-qPCR. Data reported here show that Cd is a strong inducer of MT and HSP70 genes, and that BaP is a strong inducer of P-gP and AHR genes. Exposure to Cd and BaP resulted in moderate changes in antioxidant enzymes mRNA. Since the increase of MT mRNA occured when the DNA SB level returned to its basal level, we can suggest that MT is implicated in cadmium detoxification. |
Full Text |
File |
Pages |
Size |
Access |
Author's final draft |
24 |
230 KB |
Open access |
|
6 |
874 KB |
Access on demand |
|