Isolation of 12 microsatellite markers following a pyrosequencing procedure and cross-priming in two invasive cryptic species, Alexandrium catenella (group IV) and A. tamarense (group III) (Dinophyceae)

Type Article
Date 2014-06
Language English
Author(s) Laporte Martin1, 2, Shao Zhaojun1, Berrebi Patrick1, Laabir Mohamed3, Abadie EricORCID4, Faivre Nicolas3, Rieuvilleneuve Fabien3, Masseret Estelle3
Affiliation(s) 1 : Univ Montpellier 2, UMR CNRS IRD UM2 5554, Inst Sci Evolut Montpellier, F-34095 Montpellier 5, France.
2 : Univ Laval, IBIS, Dept Biol, Quebec City, PQ G1V 0A6, Canada.
3 : Univ Montpellier 2, UMR CNRS IRD Ifremer UM2 UM1 5119, F-34095 Montpellier 5, France.
4 : Lab Environm Ressources Languedoc Roussillon Ifre, F-34203 Sete, France.
Source Marine Pollution Bulletin (0025-326X) (Pergamon-elsevier Science Ltd), 2014-06 , Vol. 83 , N. 1 , P. 302-305
DOI 10.1016/j.marpolbul.2014.04.017
WOS© Times Cited 4
Keyword(s) Invasive species, Phytoplankton, Alexandrium, Dinophyceae, Microsatellite markers, Biogeography
Abstract Alexandrium catenella (group IV) and Alexandrium tamarense (group III) (Dinophyceae) are two cryptic invasive phytoplankton species belonging to the A. tamarense species complex. Their worldwide spread is favored by the human activities, transportation and climate change. In order to describe their diversity in the Mediterranean Sea and understand their settlements and maintenances in this area, new microsatellite markers were developed based on Thau lagoon (France) samples of A. catenella and A. tamarense strains. In this study twelve new microsatellite markers are proposed. Five of these microsatellite markers show amplifications on A. tamarense and ten on A. catenella. Three of these 12 microsatellite markers allowed amplifications on both cryptic species. Finally, the haplotypic diversity ranged from 0.000 to 0.791 and 0.000 to 0.942 for A. catenella and A. tamarense respectively.
Full Text
File Pages Size Access
4 251 KB Access on demand
Author's final draft 11 113 KB Open access
Top of the page

How to cite 

Laporte Martin, Shao Zhaojun, Berrebi Patrick, Laabir Mohamed, Abadie Eric, Faivre Nicolas, Rieuvilleneuve Fabien, Masseret Estelle (2014). Isolation of 12 microsatellite markers following a pyrosequencing procedure and cross-priming in two invasive cryptic species, Alexandrium catenella (group IV) and A. tamarense (group III) (Dinophyceae). Marine Pollution Bulletin, 83(1), 302-305. Publisher's official version : https://doi.org/10.1016/j.marpolbul.2014.04.017 , Open Access version : https://archimer.ifremer.fr/doc/00189/30026/