Successful detection of pathogenic Shiga-toxin-producing Escherichia coli in shellfish, environmental waters and sediment using the ISO/TS-13136 method
| Type | Article | ||||||||||||
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| Date | 2015-04 | ||||||||||||
| Language | English | ||||||||||||
| Author(s) | Baliere Charlotte1, Rince Alain2, Thevenot Delphine3, Gourmelon Michele 1 |
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| Affiliation(s) | 1 : Unité SG2M, Département RBE, Laboratoire Santé Environnement et Microbiologie, IFREMER, Plouzané, France 2 : UCBN, U2RM Stress/Virulence EA4655, Normandie-Université, Caen, France 3 : Laboratoire LMAP-LNR, UMR5557/Equipe 6, Marcy-L'Etoile, France |
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| Source | Letters in Applied Microbiology (0266-8254) (Wiley / Blackwell), 2015-04 , Vol. 60 , N. 4 , P. 315-20 | ||||||||||||
| DOI | 10.1111/lam.12386 | ||||||||||||
| WOS© Times Cited | 14 | ||||||||||||
| Keyword(s) | eae, sediment, shellfish, Shiga-toxin-producing Escherichia coli, stx, water | ||||||||||||
| Abstract | The presence of highly pathogenic Shiga-toxin-producing Escherichia coli (STEC) in shellfish, upstream waters and sediment from coastal shellfish sites was evaluated using the ISO/TS-13136 method. Shellfish (oysters, mussels and cockles), water and sediment samples were collected monthly over a period of 1 year. The method used real-time PCR detection of stx1, stx2 and eae genes and genetic markers corresponding to the five major serogroups (O157, O26, O103, O111 and O145) on enrichment broths and the identification of STEC when these genes and markers were detected. stx genes were detected in the broth of 33% of shellfish batches (n = 126), 91% of water samples (n = 117) and 28% of sediment (n = 39). One stx1+, eae+ O26:H11 strain was isolated from a shellfish batch, and O26:H11, O145:H28 and O103:H2 strains without the stx gene (n = 9) were isolated from shellfish and waters. In conclusion, this study shows the suitability of the ISO/TS-13136 method to assess the presence of highly pathogenic E. coli strains in shellfish farming areas. It also highlights a low prevalence of STEC and consequently suggests a reduced corresponding human health risk. | ||||||||||||
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