| Type |
Article |
| Date |
2015-10 |
| Language |
English |
| Author(s) |
Ollivier Helene1, Marchant James2, Le Bayon Nicolas2, Servili Arianna 2, Claireaux Guy2 |
| Affiliation(s) |
1 : Univ Bretagne Occidentale, ORPHY, Brest, France.
2 : Ctr Ifremer Brest, LEMAR, Unite PFOM ARN, Plouzane, France. |
| Source |
Journal Of Comparative Physiology B-Biochemical Systemic And Environmental Physiology (0174-1578) (Springer Heidelberg), 2015-10 , Vol. 185 , N. 7 , P. 755-765 |
| DOI |
10.1007/s00360-015-0924-6 |
| WOS© Times Cited |
4 |
| Keyword(s) |
Sea bass, Myocyte populations, Calcium signaling, Cardiac plasticity, Inter-individual variability |
| Abstract |
Climate change challenges the capacity of fishes to thrive in their habitat. However, through phenotypic diversity, they demonstrate remarkable resilience to deteriorating conditions. In fish populations, inter-individual variation in a number of fitness-determining physiological traits, including cardiac performance, is classically observed. Information about the cellular bases of inter-individual variability in cardiac performance is scarce including the possible contribution of excitation-contraction (EC) coupling. This study aimed at providing insight into EC coupling-related Ca2+ response and thermal plasticity in the European sea bass (Dicentrarchus labrax). A cell population approach was used to lay the methodological basis for identifying the cellular determinants of cardiac performance. Fish were acclimated at 12 and 22 A degrees C and changes in intracellular calcium concentration ([Ca2+](i)) following KCl stimulation were measured using Fura-2, at 12 or 22 A degrees C-test. The increase in [Ca2+](i) resulted primarily from extracellular Ca2+ entry but sarcoplasmic reticulum stores were also shown to be involved. As previously reported in sea bass, a modest effect of adrenaline was observed. Moreover, although the response appeared relatively insensitive to an acute temperature change, a difference in Ca2+ response was observed between 12- and 22 A degrees C-acclimated fish. In particular, a greater increase in [Ca2+](i) at a high level of adrenaline was observed in 22 A degrees C-acclimated fish that may be related to an improved efficiency of adrenaline under these conditions. In conclusion, this method allows a rapid screening of cellular characteristics. It represents a promising tool to identify the cellular determinants of inter-individual variability in fishes' capacity for environmental adaptation. |
| Full Text |
| File |
Pages |
Size |
Access |
|
11 |
422 KB |
Access on demand |
| Author's final draft |
19 |
558 KB |
Open access |
|
