Characterization of 27 microsatellite loci in the European flat oyster Ostrea edulis
Type | Article | ||||||||||||||||
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Date | 2009-05 | ||||||||||||||||
Language | English | ||||||||||||||||
Author(s) | Lallias Delphine1, Stockdale Ruth1, Boudry Pierre2, Beaumont Andy R.1, Lapegue Sylvie3 | ||||||||||||||||
Affiliation(s) | 1 : Bangor Univ, Coll Nat Sci, Sch Ocean Sci, Menai Bridge LL59 5AB, Anglesey, Wales. 2 : IFREMER, UMR Physiol & Ecophysiol Mollusques Marins M100, Plouzane, France. 3 : IFREMER, Lab Genet & Pathol, F-17390 Ronce Les Bains, La Tremblade, France. |
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Source | Molecular Ecology Resources (1755-098X) (Wiley / Blackwell), 2009-05 , Vol. 9 , N. 3 , P. 960-963 | ||||||||||||||||
DOI | 10.1111/j.1755-0998.2009.02515.x | ||||||||||||||||
WOS© Times Cited | 4 | ||||||||||||||||
Keyword(s) | Universal tailed primer labelling, Oysters, Ostrea edulis, Microsatellites | ||||||||||||||||
Abstract | The flat oyster Ostrea edulis is native to Europe and populations have been severely depleted by the parasite Bonamia ostreae since the 1980s. Additional genetic markers are required to improve population genetics study and linkage map development for selection for B. ostrea-resistance in this species. Here, we characterized 27 novel microsatellite loci for O. edulis. Number of alleles per locus ranged from 6 to 25 and observed heterozygosity between 0.375 and 1. Null alleles were suggested at a few loci but most loci were in Hardy-Weinberg agreement enabling their reliable use in further population and mapping genetics approaches. | ||||||||||||||||
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