Characterization of 27 microsatellite loci in the European flat oyster Ostrea edulis

Type Article
Date 2009-05
Language English
Author(s) Lallias Delphine1, Stockdale Ruth1, Boudry PierreORCID2, Beaumont Andy R.1, Lapegue SylvieORCID3
Affiliation(s) 1 : Bangor Univ, Coll Nat Sci, Sch Ocean Sci, Menai Bridge LL59 5AB, Anglesey, Wales.
2 : IFREMER, UMR Physiol & Ecophysiol Mollusques Marins M100, Plouzane, France.
3 : IFREMER, Lab Genet & Pathol, F-17390 Ronce Les Bains, La Tremblade, France.
Source Molecular Ecology Resources (1755-098X) (Wiley / Blackwell), 2009-05 , Vol. 9 , N. 3 , P. 960-963
DOI 10.1111/j.1755-0998.2009.02515.x
WOS© Times Cited 4
Keyword(s) Universal tailed primer labelling, Oysters, Ostrea edulis, Microsatellites
Abstract The flat oyster Ostrea edulis is native to Europe and populations have been severely depleted by the parasite Bonamia ostreae since the 1980s. Additional genetic markers are required to improve population genetics study and linkage map development for selection for B. ostrea-resistance in this species. Here, we characterized 27 novel microsatellite loci for O. edulis. Number of alleles per locus ranged from 6 to 25 and observed heterozygosity between 0.375 and 1. Null alleles were suggested at a few loci but most loci were in Hardy-Weinberg agreement enabling their reliable use in further population and mapping genetics approaches.
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