Optimization of growth and bacteriocin activity of the food bioprotective Carnobacterium divergens V41 in an animal origin protein free medium
Type | Article | ||||||||
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Date | 2016-08 | ||||||||
Language | English | ||||||||
Author(s) | Brillet-Viel Anne1, Pilet Marie-France1, Courcoux Philippe2, Prevost Hervé1, Leroi Francoise3 | ||||||||
Affiliation(s) | 1 : Univ Bretagne Loire, ONIRIS, UMR 1014, SECALIM, Nantes, France 2 : Univ Bretagne Loire, ONIRIS, USC Inst Natl Rech Agron Sensometrie & Chimiometi, Nantes, France 3 : IFREMER, Lab Ecosyst Microbiens & Mol Marines Biotechnol, Nantes, France |
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Source | Frontiers in Marine Science (2296-7745) (Frontiers Media S.A), 2016-08 , Vol. 3 , N. 128 , P. 13p. | ||||||||
DOI | 10.3389/fmars.2016.00128 | ||||||||
WOS© Times Cited | 11 | ||||||||
Note | This article is part of the Research Topic Marine Biotechnology in the Food Domain Footnotes Règlement (CE) n°999/2001 du parlement européen et du conseil du 22 mai 2001 fixant les règles pour la prévention, le contrôle et l'éradication de certaines encéphalopathies spongiformes transmissibles. Journal Officiel L 147 du 31 Mai 2001. Available online at: http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=CONSLEG:2001R0999:20110318:FR:PDF (Access 25-07-2016). | ||||||||
Keyword(s) | optimization, Carnobacterium, bacteriocin, experimental design, bioreactor, Growth, Cold-smoked salmon | ||||||||
Abstract | Optimization of Carnobacterium divergens V41 growth and bacteriocin activity in a culture medium deprived of animal protein, needs for food bioprotection, was performed by using a statistical approach. In a screening experiment, twelve factors (pH, temperature, carbohydrates, NaCl, yeast extract, soy peptone, sodium acetate, ammonium citrate, magnesium sulphate, manganese sulphate, ascorbic acid and thiamine) were tested for their influence on the maximal growth and bacteriocin activity using a two-level incomplete factorial design with 192 experiments performed in microtiter plate wells. Based on results, a basic medium was developed and three variables (pH, temperature and carbohydrates concentration) were selected for a scale-up study in bioreactor. A 23 complete factorial design was performed, allowing the estimation of linear effects of factors and all the first order interactions. The best conditions for the cell production were obtained with a temperature of 15°C and a carbohydrates concentration of 20 g/l whatever the pH (in the range 6.5-8), and the best conditions for bacteriocin activity were obtained at 15°C and pH 6.5 whatever the carbohydrates concentration (in the range 2-20 g/l). The predicted final count of C. divergens V41 and the bacteriocin activity under the optimized conditions (15°C, pH 6.5, 20 g/l carbohydrates) were 2.4 x 1010 CFU/ml and 819200 AU/ml respectively. C. divergens V41 cells cultivated in the optimized conditions were able to grow in cold-smoked salmon and totally inhibited the growth of Listeria monocytogenes (< 50 CFU g-1) during five weeks of vacuum storage at 4° and 8°C. | ||||||||
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