Irradiation effects on antibody performance in the frame of biochip-based instruments development for space exploration

Type Article
Date 2017-01
Language English
Author(s) Baque M.1, Dobrijevic M.2, 3, Le Postollec A.2, 3, Moreau T.4, Faye C.5, Vigier F., Incerti S.6, 7, Coussot G.8, Caron J.9, Vandenabeele Trambouze Odile10, 11, 12
Affiliation(s) 1 : Univ Roma Tor Vergata, Dept Biol, Rome, Italy.
2 : Univ Bordeaux, LAB, UMR 5804, F-33270 Florac, France.
3 : CNRS, LAB, UMR 5804, F-33270 Florac, France.
4 : Univ Clermont Ferrand, INSERM U1103, GReD, CNRS UMR6293, Aubiere, France.
5 : Cap Alpha, COLCOM, F-34830 Clapiers, France.
6 : Univ Bordeaux, CENBG, UMR 5797, F-33170 Gradignan, France.
7 : CNRS, IN2P3, CENBG, UMR 5797, F-33170 Gradignan, France.
8 : Univ Montpellier, Inst Biomol Max Mousseron, Fac Pharm, CNRS,Unite Mixte Rech 5247, F-34093 Montpellier 5, France.
9 : Inst Bergonie, Dept Radiotherapy, Ctr Comprehens Canc, F-33076 Bordeaux, France.
10 : Univ Bretagne Occidentale, LMEE, UEB, IUEM UMR 6197, Plouzane, France.
11 : CNRS, IUEM UMR 6197, LMEE, Plouzane, France.
12 : IFREMER, UMR6197, LMEE, Plouzane, France.
Source International Journal Of Astrobiology (1473-5504) (Cambridge Univ Press), 2017-01 , Vol. 16 , N. 1 , P. 82-90
DOI 10.1017/S1473550415000555
WOS© Times Cited 12
Keyword(s) astrobiology, biochip, cosmic rays, search for extraterrestrial life
Abstract Several instruments based on immunoassay techniques have been proposed for life-detection experiments in the framework of planetary exploration but few experiments have been conducted so far to test the resistance of antibodies against cosmic ray particles. We present several irradiation experiments carried out on both grafted and free antibodies for different types of incident particles (protons, neutrons, electrons and C-12) at different energies (between 9 MeV and 50 MeV) and different fluences. No loss of antibodies activity was detected for the whole set of experiments except when considering protons with energy between 20 and 30 MeV (on free and grafted antibodies) and fluences much greater than expected for a typical planetary mission to Mars for instance. Our results on grafted antibodies suggest that biochip-based instruments must be carefully designed according to the expected radiation environment for a given mission. In particular, a surface density of antibodies much larger than the expected proton fluence would prevent significant loss of antibodies activity and thus assuring a successful detection.
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Baque M., Dobrijevic M., Le Postollec A., Moreau T., Faye C., Vigier F., Incerti S., Coussot G., Caron J., Vandenabeele Trambouze Odile (2017). Irradiation effects on antibody performance in the frame of biochip-based instruments development for space exploration. International Journal Of Astrobiology, 16(1), 82-90. Publisher's official version : https://doi.org/10.1017/S1473550415000555 , Open Access version : https://archimer.ifremer.fr/doc/00366/47678/