FN Archimer Export Format PT J TI Highlighting of quorum sensing lux genes and their expression in the hydrothermal vent shrimp Rimicaris exoculata ectosymbiontic community. Possible use as biogeographic markers BT AF LE BLOA, Simon DURAND, Lucile CUEFF-GAUCHARD, Valerie LE BARS, Josiane TAUPIN, Laure MARTEAU, Charlotte BAZIRE, Alexis CAMBON-BONAVITA, Marie-Anne AS 1:1,2,3;2:1;3:1,2,3;4:1,2,3;5:4;6:4;7:4;8:1,2,3; FF 1:PDG-REM-EEP-LMEE;2:PDG-REM-EEP-LMEE;3:PDG-REM-EEP-LMEE;4:PDG-REM-EEP-LMEE;5:;6:;7:;8:PDG-REM-EEP-LMEE; C1 IFREMER, Lab Microbiol Environm Extremes, UMR Ifremer CNRS UBO 6197, Ctr Bretagne,REM EEP LM2E, Plouzane, France. Univ Brest, Lab Microbiol Environm Extremes, UMR Ifremer CNRS UBO 6197, Technopole Iroise,4 Pl Nicolas Copernic, Plouzane, France. CNRS, Lab Microbiol Environm Extremes, UMR Ifremer CNRS UBO 6197, Technopole Iroise,4 Pl Nicolas Copernic, Plouzane, France. Univ Bretagne Sud, EA 3884, LBCM, Rue St Maude, Lorient, France. C2 IFREMER, FRANCE UBO, FRANCE CNRS, FRANCE UBS, FRANCE SI BREST SE PDG-REM-EEP-LMEE UM BEEP-LM2E IN WOS Ifremer jusqu'en 2018 DOAJ copubli-france copubli-univ-france IF 2.766 TC 7 UR https://archimer.ifremer.fr/doc/00377/48841/49268.pdf https://archimer.ifremer.fr/doc/00377/48841/49269.pdf https://archimer.ifremer.fr/doc/00377/48841/49270.tif https://archimer.ifremer.fr/doc/00377/48841/49271.tif https://archimer.ifremer.fr/doc/00377/48841/49272.tif LA English DT Article CR BICOSE BIOBAZ 2013 SERPENTINE BO Pourquoi pas ? AB Rimicaris exoculata is a caridean shrimp that dominates the fauna at several hydrothermal vent sites of the Mid-Atlantic Ridge. It has two distinct and stable microbial communities. One of these epibiontic bacterial communities is located in the shrimp gut and has a distribution and role that are poorly understood. The second colonizes its enlarged gill chamber and is involved in host nutrition. It is eliminated after each molt, and has colonization processes reminiscent of those of a biofilm. The presence and expression of genes usually involved in quorum sensing (QS) were then studied. At four sites, Rainbow, TAG, Snake Pit and Logatchev, two lux genes were identified in the R. exoculata epibiontic community at different shrimp molt stages and life stages. RT-PCR experiments highlighted lux gene expression activity at TAG, Snake Pit and Rainbow vent sites. Their potential QS activity and their possible roles in epibiont colonization processes are discussed. Moreover, phylogenetic analysis has shown the presence of three clades for luxS (Epsilonproteobacteria) and four clades for luxR (Gammaproteobacteria) genes, each clade being restricted to a single site. These genes are more divergent than the 16S rRNA one. They could therefore be used as biogeographical genetic markers. PY 2017 PD MAR SO Plos One SN 1932-6203 PU Public Library Science VL 12 IS 3 UT 000399094700083 DI 10.1371/journal.pone.0174338 ID 48841 ER EF