Development of a duplex Taqman real-time PCR assay for rapid identification of Vibrio splendidus -related and V. aestuarianus strains from bacterial cultures
| Type | Article | ||||||||||||
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| Date | 2017-09 | ||||||||||||
| Language | English | ||||||||||||
| Author(s) | Saulnier Denis1, de Decker Sophie2, Tourbiez Delphine2, Travers Marie-Agnes 2 |
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| Affiliation(s) | 1 : IFREMER, UMR EIO 241, UPF ILM IRD, BP 49, F-98719 Tahiti, French Polynesi, France. 2 : IFREMER, RBE SG2M, Lab Genet & Pathol Mollusques Marins, Av Mus de Loup, F-17390 La Tremblade, French Polynesi, France. |
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| Source | Journal Of Microbiological Methods (0167-7012) (Elsevier Science Bv), 2017-09 , Vol. 140 , P. 67-69 | ||||||||||||
| DOI | 10.1016/j.mimet.2017.07.002 | ||||||||||||
| WOS© Times Cited | 23 | ||||||||||||
| Keyword(s) | Multiplex real-time PCR, Gene sequencing, Molluscs, Splendidus Glade, Vibrio aestuarianus | ||||||||||||
| Abstract | To enable the rapid and accurate identification of Vibrio splendidus-related and V. aestuarianus strains associated with Pacific cupped oyster Crassostrea gigas mortality, we developed a duplex Taqman real-time PCR assay and evaluated its efficacy. This technique proved to be rapid, sensitive, and specific and will be particularly valuable for epidemiologic studies. |
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