FN Archimer Export Format
PT J
TI Validity of Escherichia coli, enterovirus, and F-specific RNA bacteriophages as indicators of viral shellfish contamination
BT 
AF MIOSSEC, Laurence
   LE GUYADER, Soizick
   PELLETIER, Dominique
   HAUGARREAU, Larissa
   CAPRAIS, Marie-Paule
   POMMEPUY, Monique
AS 1:1;2:1;3:2;4:1;5:3;6:3;
FF 1:PDG-DRV-RA-LGP;2:PDG-DEL-MP-MIC;3:PDG-DRV-RH-MAERHA;4:PDG-DEL-MP-MIC;5:PDG-DEL-MP-MIC;6:PDG-DEL-MP-MIC;
C1 IFREMER, Microbiol Lab, F-44311 Nantes 3, France.
   IFREMER, Lab Math Appl Exploitat Ressources Halieutiques &, F-44311 Nantes, France.
   IFREMER, Microbiol Lab, F-29280 Plouzane, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
SI LA TREMBLADE
   NANTES
   BREST
SE PDG-DRV-RA-LGP
   PDG-DEL-MP-MIC
   PDG-DRV-RH-MAERHA
IN WOS Ifremer jusqu'en 2018
IF 0.528
TC 10
UR https://archimer.ifremer.fr/doc/00000/10006/7794.pdf
LA English
DT Article
DE ;indicator;Escherichia coli;F specific RNA bacteriophages;enteric viruses;viral contamination;shellfish
AB The sanitary classification of harvesting areas for bivalve mollusks in France is based on the level of Escherichia Coli contamination detected in shellfish meat, as defined in EC Directive 91/492 EEC. However, outbreaks of gastroenteritis or hepatitis after consumption of shellfish meeting current bacteriological standards suggest that E. coli is a poor indicator of viral contamination. The purpose of this study was to assess the adequacy of enterovirus and F-specific RNA bacteriophages as new indicators of human enteric viruses. Shellfish were sampled over a 37-mo period to characterize microbial contamination in two coastal areas subjected to different sewage contamination inputs. Contamination by E. coli, F-specific RNA bacteriophages (F+ RNA) and human enteric viruses (enterovirus, EV; hepatitis A virus, HAV; Norwalk-like virus, NLV: astrovirus, AV; and rotavirus, RV) was measured in the same samples. E. coli analysis was performed by conductance measurement, enteric viruses were detected by reverse-transcription polymerase chain reaction (RT-PCR) and hybridization. and F+ RNA was evaluated by culture according to the ISO 10705-1 method. Statistical analysis based on bootstrap methods was performed on 95 series of paired observations. The validity of E. coli, enterovirus, and F-specific RNA bacteriophages as viral indicators was evaluated by measuring their sensitivity and specificity in the presence of enteric viruses. None of the tested indicators proved adequate to protect the public from viral shellfish contamination. The sensitivity of all indicators was better in the highly contaminated zone, and enteroviruses showed the highest specificity for both sites. 
PY 2001
PD DEC
SO Journal Of Shellfish Research
SN 0730-8000
PU Natl Shellfisheries Assoc
VL 20
IS 3
UT 000174127300039
BP 1223
EP 1227
ID 10006
ER

EF