FN Archimer Export Format
PT J
TI Effects of extracellular products from the pathogenic Vibrio aestuarianus strain 01/32 on lethality and cellular immune responses of the oyster Crassostrea gigas
BT 
AF LABREUCHE, Yannick
   SOUDANT, Philippe
   GONCALVES, Madeleine
   LAMBERT, Christophe
   NICOLAS, Jean-Louis
AS 1:2;2:1;3:1;4:1;5:2;
FF 1:PDG-DOP-DCB-PFOM-PI;2:;3:;4:;5:PDG-DOP-DCB-PFOM-PI;
C1 Univ Bretagne Occidentale, Inst Univ Europeen de la Mer, Lab Sci Environm Marin, F-29280 Plouzane, France.
   IFREMER, Ctr Brest, Unite Mixte Rech Physiol & Ecophysiol Mollusques, F-29280 Plouzane, France.
C2 UBO, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-DOP-DCB-PFOM-PI
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 3.399
TC 109
UR https://archimer.ifremer.fr/doc/2006/publication-1035.pdf
LA English
DT Article
DE ;Vibrio aestuarianus;ECPs;Crassostrea gigas;ROS production;Adhesion;Phagocytosis;Flow cytometry;Bivalve immunity
AB Vibrio aestuarianus strain 01/32 was previously shown to be pathogenic to Crassostrea gigas juveniles. To investigate virulence mechanisms of this pathogen, we studied the toxicity to oysters of its extracellular products (ECPs). ECPs displayed lethality to animals, with a LD50 value of 3.3 mu g/g body weight. To determine the oyster cellular immune responses induced by these ECPs, we further examined in vitro their effects on C. gigas hemocytes, using flow cytometric-based hemocyte assays. Treatment of hemolymph with ECPs caused a significant inhibition of hemocyte phagocytosis and adhesive capabilities. In contrast, the pathway of reactive oxygen species production was enhanced by higher ECP concentrations. Exposure of hemocytes to live bacteria induced no changes in hemocyte parameters. Together, these results suggest that V. aestuarianus strain 01/32 secretes one or more factors which may play an important role in the pathogenicity of this microorganism, and which display immunosuppressant activities on hemocyte functions. (c) 2005 Elsevier Ltd. All fights reserved.
PY 2006
SO Developmental & Comparative Immunology
SN 0145-305X
PU Elsevier
VL 30
IS 4
UT 000234870900003
BP 367
EP 379
DI 10.1016/j.dci.2005.05.003
ID 1035
ER

EF