FN Archimer Export Format PT J TI Toxic factors of Vibrio strains pathogenic to shrimp BT AF GOARANT, Cyrille HERLIN, Jose BRIZARD, Raphael MARTEAU, Anne-Laure MARTIN, C MARTIN, B AS 1:1;2:1;3:1;4:1;5:2;6:2; FF 1:PDG-DRV-RA-NC;2:PDG-DRV-RA-NC;3:PDG-DRV-RA-LGP;4:;5:;6:; C1 IFREMER, Lab Rech Aquacole, Stn Aquaculture St Vincent, Noumea 98845, New Caledonia. Univ Francaise Pacifique, Ctr Univ Polynesie Francaise, Biol Cellulaire Lab, Tahiti, Fr Polynesia. C2 IFREMER, FRANCE UNIV FRANCAISE PACIFIQUE, FRANCE SI SAINT VINCENT LA TREMBLADE SE PDG-DRV-RA-NC PDG-DRV-RA-LGP PDG-RBE-LEADNC-LAC IN WOS Ifremer jusqu'en 2018 copubli-univ-france IF 1.556 TC 34 UR https://archimer.ifremer.fr/doc/00000/10543/7600.pdf LA English DT Article DE ;vibrio;Vibrio penaeicida;shrimp;extracellular products;temperature AB Vibriosis is a major disease problem in shrimp aquaculture. 'Syndrome 93' is a seasonal juvenile vibriosis caused by Vibrio penaeicida which affects Litopenaeus stylirostris in grow-out ponds in New Caledonia. This study assessed the toxic activities of extracellular products (ECPs) from V: penaeicida, V. alginolyticus and V, nigripulchritudo using in vivo injections in healthy juvenile L. stylirostris (= Penaeus stylirostris) and in vitro assays on shrimp primary cell cultures and the fish cell line epithelioma papulosum cyprini (EPC). Toxic effects of ECPs were demonstrated for all pathogenic Vibrio strains tested both in vivo and in vitro, but for shrimp only; no effect was observed on the fish cell line. ECP toxicity for New Caledonian V. penaeicida was found only after cultivation at low temperature (20 degrees C) and not at higher temperature (30 degrees C). This points to the fact that 'Syndrome 93' episodes are triggered by temperature drops. The assays used here demonstrate the usefulness of primary shrimp cell cultures to study virulence mechanisms of shrimp pathogenic bacteria. PY 2000 PD MAR SO Diseases Of Aquatic Organisms SN 0177-5103 PU Inter-research VL 40 IS 2 UT 000086771500003 BP 101 EP 107 DI 10.3354/dao040101 ID 10543 ER EF