|Author(s)||Choquet Gwenaelle, Soudant P, Lambert C, Nicolas Jean-Louis, Paillard C|
|Affiliation(s)||IUEM, UBO, Lab Sci Environm Marin, LEMAR, F-29280 Plouzane, France.
IFREMER, Ctr Brest, LPI, F-29280 Plouzane, France.
|Source||Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 2003-12 , Vol. 57 , N. 1-2 , P. 109-116|
|WOS© Times Cited||62|
|Keyword(s)||cytotoxicity, flow cytometry, Vibrio tapetis, Ruditapes philippinarum, bivalve hemocytes, pathogenicity|
|Abstract||Vibrio tapetis is the causative agent of brown ring disease (BRD), which affects a species of clam, Ruditapes philippinarum. After incubation with V. tapetis, hemocytes lose filopods and become rounded, indicating cytotoxic activity of the bacterium. To rapidly quantify this cytotoxicity, a flow-cytometry test was developed based on the capacity of V tapetis to inhibit adhesion of clam hemocytes to plastic. Several bacteria:hemocyte ratios, the cytotoxicity of other Vibrio spp. pathogenic to bivalves, and that of various V. tapetis isolates were tested. Inhibition of adherence is detectable with as few as 5 bacteria per hemocyte. The greater cytotoxic activity of V tapetis compared to that of V splendidus and V pectenicida suggests a specific pathogenicity of V. tapetis to R. philippinarum hemocytes. Although all V. tapetis isolates inhibited adhesion, significant variations in cytotoxicity among isolates was demonstrated.|
Choquet Gwenaelle, Soudant P, Lambert C, Nicolas Jean-Louis, Paillard C (2003). Reduction of adhesion properties of Ruditapes philippinarum hemocytes exposed to Vibrio tapetis. Diseases Of Aquatic Organisms, 57(1-2), 109-116. Publisher's official version : https://doi.org/10.3354/dao057109 , Open Access version : https://archimer.ifremer.fr/doc/00000/10761/