| Type |
Article |
| Date |
2003-12 |
| Language |
English |
| Author(s) |
Choquet Gwenaelle, Soudant P, Lambert C, Nicolas Jean-Louis, Paillard C |
| Affiliation(s) |
IUEM, UBO, Lab Sci Environm Marin, LEMAR, F-29280 Plouzane, France.
IFREMER, Ctr Brest, LPI, F-29280 Plouzane, France. |
| Source |
Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 2003-12 , Vol. 57 , N. 1-2 , P. 109-116 |
| DOI |
10.3354/dao057109 |
| WOS© Times Cited |
62 |
| Keyword(s) |
cytotoxicity, flow cytometry, Vibrio tapetis, Ruditapes philippinarum, bivalve hemocytes, pathogenicity |
| Abstract |
Vibrio tapetis is the causative agent of brown ring disease (BRD), which affects a species of clam, Ruditapes philippinarum. After incubation with V. tapetis, hemocytes lose filopods and become rounded, indicating cytotoxic activity of the bacterium. To rapidly quantify this cytotoxicity, a flow-cytometry test was developed based on the capacity of V tapetis to inhibit adhesion of clam hemocytes to plastic. Several bacteria:hemocyte ratios, the cytotoxicity of other Vibrio spp. pathogenic to bivalves, and that of various V. tapetis isolates were tested. Inhibition of adherence is detectable with as few as 5 bacteria per hemocyte. The greater cytotoxic activity of V tapetis compared to that of V splendidus and V pectenicida suggests a specific pathogenicity of V. tapetis to R. philippinarum hemocytes. Although all V. tapetis isolates inhibited adhesion, significant variations in cytotoxicity among isolates was demonstrated. |
| Full Text |
| File |
Pages |
Size |
Access |
| 7590.pdf |
8 |
228 KB |
Open access |
|
