FN Archimer Export Format
PT J
TI Reduction of adhesion properties of Ruditapes philippinarum hemocytes exposed to Vibrio tapetis
BT 
AF CHOQUET, Gwenaelle
   SOUDANT, P
   LAMBERT, C
   NICOLAS, Jean-Louis
   PAILLARD, C
AS 1:;2:;3:;4:;5:;
FF 1:PDG-DRV-RA-PI;2:;3:;4:PDG-DRV-RA-PI;5:;
C1 IUEM, UBO, Lab Sci Environm Marin, LEMAR, F-29280 Plouzane, France.
   IFREMER, Ctr Brest, LPI, F-29280 Plouzane, France.
C2 UNIV BRETAGNE OCCIDENTALE, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-DRV-RA-PI
IN WOS Ifremer jusqu'en 2018
IF 1.263
TC 62
UR https://archimer.ifremer.fr/doc/00000/10761/7590.pdf
LA English
DT Article
DE ;cytotoxicity;flow cytometry;Vibrio tapetis;Ruditapes philippinarum;bivalve hemocytes;pathogenicity
AB Vibrio tapetis is the causative agent of brown ring disease (BRD), which affects a species of clam, Ruditapes philippinarum. After incubation with V. tapetis, hemocytes lose filopods and become rounded, indicating cytotoxic activity of the bacterium. To rapidly quantify this cytotoxicity, a flow-cytometry test was developed based on the capacity of V tapetis to inhibit adhesion of clam hemocytes to plastic. Several bacteria:hemocyte ratios, the cytotoxicity of other Vibrio spp. pathogenic to bivalves, and that of various V. tapetis isolates were tested. Inhibition of adherence is detectable with as few as 5 bacteria per hemocyte. The greater cytotoxic activity of V tapetis compared to that of V splendidus and V pectenicida suggests a specific pathogenicity of V. tapetis to R. philippinarum hemocytes. Although all V. tapetis isolates inhibited adhesion, significant variations in cytotoxicity among isolates was demonstrated.
PY 2003
PD DEC
SO Diseases Of Aquatic Organisms
SN 0177-5103
PU Inter-research
VL 57
IS 1-2
UT 000189277700014
BP 109
EP 116
DI 10.3354/dao057109
ID 10761
ER

EF