FN Archimer Export Format PT J TI Physiology and continuous culture of the hyperthermophilic deep-sea vent archaeon Pyrococcus abyssi ST549 BT AF GODFROY, Anne RAVEN, N SHARP, R AS 1:;2:;3:; FF 1:PDG-DRV-VP-MBE;2:;3:; C1 IFREMER, DRV VP, Lab Caracterisat Microorganismes, F-29280 Plouzane, France. Publ Hlth Lab Serv, Ctr Appl Microbiol & Res, Salisbury SP4 0JG, Wilts, England. C2 IFREMER, FRANCE PUBL HLTH LAB SERV, ENGLAND SI BREST SE PDG-DRV-VP-MBE IN WOS Ifremer jusqu'en 2018 copubli-europe IF 1.615 TC 24 UR https://archimer.ifremer.fr/doc/2000/publication-1087.pdf LA English DT Article DE ;Sulfur requirement;Continuous culture;Nutrition;Hyperthermophile;Pyrococcus abyssi AB The deep-sea vent archaeon Pyrococcus abyssi strain ST549 was grown in batch cultures in closed bottles and by continuous culture in a gas-lift bioreactor, both in the presence and in the absence of elemental sulfur. Growth on carbohydrates, proteinaceous substrates and amino acids was investigated. The disaccharides maltose and cellobiose were shown not to be able to enhance growth suggesting that P. abyssi ST549 is unable to use them as carbon sources. By contrast, proteinaceous substrates such as peptone and brain heart infusion were shown to be very good substrates for the growth of P. abyssi ST549 and allowed growth at high steady-state cell densities in continuous culture. Growth on brain heart infusion was shown to require additional nutrients when sulfur was not present in the culture medium. Growth on amino acids only took place in the presence of sulfur. These results indicate that sulfur plays an important role in the metabolism and energetics of P. abyssi ST549. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. PY 2000 PD MAY SO FEMS Microbiology Letters SN 0378-1097 PU Elsevier VL 186 IS 1 UT 000086744500020 BP 127 EP 132 DI 10.1016/S0378-1097(00)00130-0 ID 1087 ER EF