FN Archimer Export Format
PT J
TI Comparative assessment of human and farm animal faecal microbiota using real-time quantitative PCR
BT 
AF FURET, Jean-Pierre
   FIRMESSE, Olivier
   GOURMELON, Michele
   BRIDONNEAU, Chantal
   TAP, Julien
   MONDOT, Stanislas
   DORE, Joel
   CORTHIER, Gerard
AS 1:1;2:1;3:2;4:1;5:1;6:1;7:1;8:1;
FF 1:;2:;3:PDG-DOP-DCN-EMP-MIC;4:;5:;6:;7:;8:;
C1 INRA, Unite Ecol & Physiol Syst Digestif, U910, F-78350 Jouy En Josas, France.
   IFREMER, Microbiol Lab, EMP, MIC, Plouzane, France.
C2 INRA, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-DOP-DCN-EMP-MIC
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
IF 3.598
TC 262
UR https://archimer.ifremer.fr/doc/00000/11071/11297.pdf
LA English
DT Article
DE ;quantitative PCR;faecal microbiota;human;farm animals
AB Pollution of the environment by human and animal faecal pollution affects the safety of shellfish, drinking water and recreational beaches. To pinpoint the origin of contaminations, it is essential to define the differences between human microbiota and that of farm animals. A strategy based on real-time quantitative PCR (qPCR) assays was therefore developed and applied to compare the composition of intestinal microbiota of these two groups. Primers were designed to quantify the 16S rRNA gene from dominant and subdominant bacterial groups. TaqMan((R)) probes were defined for the qPCR technique used for dominant microbiota. Human faecal microbiota was compared with that of farm animals using faecal samples collected from rabbits, goats, horses, pigs, sheep and cows. Three dominant bacterial groups (Bacteroides/Prevotella, Clostridium coccoides and Bifidobacterium) of the human microbiota showed differential population levels in animal species. The Clostridium leptum group showed the lowest differences among human and farm animal species. Human subdominant bacterial groups were highly variable in animal species. Partial least squares regression indicated that the human microbiota could be distinguished from all farm animals studied. This culture-independent comparative assessment of the faecal microbiota between humans and farm animals will prove useful in identifying biomarkers of human and animal faecal contaminations that can be applied to microbial source tracking methods.
PY 2009
PD JUL
SO Fems Microbiology Ecology
SN 0168-6496
PU Wiley-blackwell Publishing, Inc
VL 68
IS 3
UT 000265770900009
BP 351
EP 362
DI 10.1111/j.1574-6941.2009.00671.x
ID 11071
ER

EF