FN Archimer Export Format
PT J
TI Microbial diversity associated with the hydrothermal shrimp Rimicaris exoculata gut and occurrence of a resident microbial community
BT 
AF DURAND, Lucile
   ZBINDEN, Magali
   CUEFF-GAUCHARD, Valerie
   DUPERRON, Sebastien
   ROUSSEL, Erwan
   SHILLITO, Bruce
   CAMBON-BONAVITA, Marie-Anne
AS 1:1;2:2;3:1;4:2;5:1;6:2;7:1;
FF 1:PDG-DOP-DCB-EEP-LMEE;2:;3:PDG-DOP-DCB-EEP-LMEE;4:;5:PDG-DOP-DCB-EEP-LMEE;6:;7:PDG-DOP-DCB-EEP-LMEE;
C1 IFREMER, Ctr Brest, Lab Microbiol Environm Extremes, DEEP LM2E,UMR 6197, F-29280 Plouzane, France.
   Univ Paris 06, CNRS, UMR 7138, Paris, France.
C2 IFREMER, FRANCE
   UNIV PARIS 06, FRANCE
SI BREST
SE PDG-DOP-DCB-EEP-LMEE
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 3.456
TC 63
TU Centre national de la recherche scientifique
   Institut de recherche pour le développement
   Institut français de recherche pour l'exploitation de la mer
   Muséum national d'histoire naturelle
   Université Nice - Sophia-Antipolis
   Université Pierre et Marie Curie
   Université de Bretagne Occidentale
   Université des Antilles
UR https://archimer.ifremer.fr/doc/00000/11142/7919.pdf
LA English
DT Article
CR EXOMAR
   MOMAR2008-LEG2
   MOMARDREAM-NAUT1-NAUT2
BO Unknown
   L'Atalante
   Pourquoi pas ?
DE ;Deferribacteres;midgut epibiosis;Mollicutes;Proteobacteria;Rimicaris exoculata;starvation experiment
AB Rimicaris exoculata dominates the megafauna of several Mid-Atlantic Ridge hydrothermal sites. Its gut is full of sulphides and iron-oxide particles and harbours microbial communities. Although a trophic symbiosis has been suggested, their role remains unclear. In vivo starvation experiments in pressurized vessels were performed on shrimps from Rainbow and Trans-Atlantic Geotraverse sites in order to expel the transient gut contents. Microbial communities associated with the gut of starved and reference shrimps were compared using 16S rRNA gene libraries and microscopic observations (light, transmission and scanning electron microscopy and FISH analyses). We show that the gut microbiota of shrimps from both sites included mainly Deferribacteres, Mollicutes, Epsilon- and Gammaproteobacteria. For the first time, we have observed filamentous bacteria, inserted between microvilli of gut epithelial cells. They remained after starvation periods in empty guts, suggesting the occurrence of a resident microbial community. The bacterial community composition was the same regardless of the site, except for Gammaproteobacteria retrieved only in Rainbow specimens. We observed a shift in the composition of the microbiota of long-starved specimens, from the dominance of Deferribacteres to the dominance of Gammaproteobacteria. These results reinforce the hypothesis of a symbiotic relationship between R. exoculata and its gut epibionts.
PY 2010
PD FEB
SO Fems Microbiology Ecology
SN 0168-6496
PU Wiley-blackwell Publishing, Inc
VL 71
IS 2
UT 000273065000012
BP 291
EP 303
DI 10.1111/j.1574-6941.2009.00806.x
ID 11142
ER

EF