FN Archimer Export Format PT J TI Dietary vitamin D-3 affects digestive system ontogenesis and ossification in European sea bass (Dicentrachus labrax, Linnaeus, 1758) BT AF DARIAS, Maria J. MAZURAIS, David KOUMOUNDOUROS, Giorgos GLYNATSI, Nomiki CHRISTODOULOPOULOU, Stavroula HUELVAN, Christine DESBRUYERES, Elisabeth LE GALL, Marie-Madeleine QUAZUGUEL, Patrick CAHU, Chantal ZAMBONINO-INFANTE, Jose L. AS 1:1;2:1;3:2;4:2;5:2;6:1;7:1;8:1;9:1;10:1;11:1; FF 1:;2:PDG-DOP-DCB-PFOM-ARN;3:;4:;5:;6:PDG-DOP-DCB-PFOM-ARN;7:PDG-DOP-DCB-PFOM-ARN;8:PDG-DOP-DCB-PFOM-ARN;9:PDG-DOP-DCB-PFOM-ARN;10:PDG-DOP-DCB-PFOM;11:; C1 IFREMER, Ifremer Marine Fish Nutr Team, UMR 1067, Nutr Aquaculture & Genom Res Unit, F-29280 Plouzane, France. Univ Patras, Dept Biol, Patras 26500, Greece. C2 IFREMER, FRANCE UNIV PATRAS, GREECE SI BREST SE PDG-DOP-DCB-PFOM-ARN PDG-DOP-DCB-PFOM IN WOS Ifremer jusqu'en 2018 copubli-europe IF 2.044 TC 44 UR https://archimer.ifremer.fr/doc/00000/11147/7849.pdf LA English DT Article DE ;Fish larvae;Ossification;Malformations;Vitamin D;Digestive system;Gene expression AB The influence of dietary vitamin D-3 (VD3) on survival, growth and morphogenesis during the larval development of European sea bass (Dicentrarchus labrax) was evaluated until 45 days post hatching. Diets contained 4% of the standard vitamin mix (VM) recommended by the National Research Council (NRC) and incorporated 0, 19.2, 38.4, or 140 IU of VD3 per gram of diet to give VD-0, VD-1, VD-2 and VD-3 dietary treatments, respectively. The present study revealed for the first time an impact of dietary VD3 on the sea bass digestive system ontogenesis that consequently conditioned the ossification process and morphogenesis. All dietary VD3 levels were in the "adequate range" based on larval survival. Nevertheless, growth, intestinal maturation and ossification at the end of the larval stage were harmed by the minimum dose of VD3 tested and resulted in the appearance of malformations. VD-2 and VD-3 groups showed satisfactory growth and ossification levels at the end of the larval period. However, results of enzymatic activity and expression of genes involved in the VD3 pathway (bone morphogenetic protein 4, osteocalcin, vitamin D receptors and transient receptor potential cation channel-subfamily V. member 6-) gave evidence of complications during the ossification process as revealed by the high percentage of deformed larvae. A VD3 level of 19.2 IU/g diet appeared necessary to obtain harmonious larval morphogenesis. (C) 2009 Elsevier B.V. All rights reserved. PY 2010 PD JAN SO Aquaculture SN 0044-8486 PU Elsevier Science Bv VL 298 IS 3-4 UT 000273917800016 BP 300 EP 307 DI 10.1016/j.aquaculture.2009.11.002 ID 11147 ER EF