FN Archimer Export Format
PT J
TI One step immunochromatographic assay for the rapid detection of Alexandrium minutum
BT 
AF GAS, Fabienne
   BAUS, Beatrice
   PINTO, Laetitia
   COMPERE, Chantal
   TANCHOU, Valerie
   QUEMENEUR, Eric
AS 1:1;2:1;3:1;4:2;5:1;6:1;
FF 1:;2:;3:;4:PDG-DOP-DCB-RDT-IC;5:;6:;
C1 CEA Marcoule, Direct Sci Vivant, iBEB SBTN LDCAE, Bagnols Sur Ceze, France.
   IFREMER, Ctr Brest, Serv Interfaces & Capteurs, Plouzane, France.
C2 CEA, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-DOP-DCB-RDT-IC
IN WOS Ifremer jusqu'en 2018
   copubli-france
IF 5.361
TC 24
UR https://archimer.ifremer.fr/doc/00000/11153/7819.pdf
LA English
DT Article
DE ;Antibody;Alexandrium minutum;Immunochromatographic assay (ICA);Rapid detection
AB Harmful algal blooms represent a major threat to marine production, and particularly to shellfish farming. Current methods for analyzing environmental samples are tedious and time consuming because they require taxonomists and animal experiments. New rapid detection methods, such as immunoassays, are sought for alerting purposes and for the study of algal ecodynamics in their natural environment. Alexandrium minutum, which causes paralytic shellfish poisoning, occurs with increasing frequency along European coasts. We have developed a one step immunochromatographic assay which is based on the principle of immunochromatographic analysis and involves the use of two distinct monoclonal antibodies directed against surface antigens of A. minutum. The primary specific antibody was conjugated with colloidal gold, and the secondary antibody (capture reagent) is immobilized on a strip of nitrocellulose membrane. We could demonstrate that whole algae are able to diffuse without restriction in the porous material. The assay time for this qualitative but highly specific assay was less than 15 min, suitable for rapid on-site testing. (C) 2009 Elsevier B.V. All rights reserved.
PY 2010
SO Biosensors & Bioelectronics
SN 0956-5663
PU Elsevier Advanced Technology
VL 25
IS 5
UT 000273945800046
BP 1235
EP 1239
DI 10.1016/j.bios.2009.09.033
ID 11153
ER

EF