FN Archimer Export Format PT J TI Use of rotavirus virus-like particles as surrogates to evaluate virus persistence in shellfish BT AF LOISY, Fabienne ATMAR, R LE SAUX, Jean-Claude COHEN, J CAPRAIS, Marie-Paule POMMEPUY, Monique LE GUYADER, Soizick AS 1:1;2:2;3:1;4:3;5:1;6:1;7:1; FF 1:PDG-DOP-DCN-EMP-MIC;2:;3:PDG-DOP-DCN-EMP-MIC;4:;5:PDG-DOP-DCN-EMP-MIC;6:PDG-DOP-DCN-EMP;7:PDG-DOP-DCN-EMP-MIC; C1 IFREMER, Microbiol Lab, F-44311 Nantes, France. Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA. CNRS, INRA, Unite Mixte, F-91198 Gif Sur Yvette, France. C2 IFREMER, FRANCE BAYLOR COLL MED, USA CNRS, FRANCE SI NANTES BREST SE PDG-DOP-DCN-EMP-MIC PDG-DOP-DCN-EMP IN WOS Ifremer jusqu'en 2018 copubli-france copubli-int-hors-europe IF 3.818 TC 57 UR https://archimer.ifremer.fr/doc/2005/publication-1233.pdf LA English DT Article DE ;Seawater;Viral diseases;Bioaccumulation;Depuration;Contamination;Model;Pancreas;Regression analysis;Shellfish;Phages;Virus AB Rotavirus virus-like particles (VLPs) and MS2 bacteriophages were bioaccumulated in bivalve mollusks to evaluate viral persistence in shellfish during depuration and relaying under natural conditions. Using this nonpathogenic surrogate virus, we were able to demonstrate that about 1 log(10) of VLPs was depurated after 1 week in warm seawater (22 degrees C). Phage MS2 was depurated more rapidly (about 2 log(10) in 1 week) than were VLPs, as determined using a single-compartment model and linear regression analysis. After being relayed in the estuary under the influence of the tides, VLPs were detected in oysters for up to 82 days following seeding with high levels of VLPs (concentration range between 10(10) and 10(9) particles per g of pancreatic tissue) and for 37 days for lower contamination levels (10(5) particles per g of pancreatic tissue). These data suggest that viral particles may persist in shellfish tissues for several weeks. PY 2005 PD OCT SO Applied and environmental microbiology SN 0099-2240 PU American society for microbiology VL 71 IS 10 UT 000232504000047 BP 6049 EP 6053 DI 10.1128/AEM.71.10.6049-6053.2005 ID 1233 ER EF