FN Archimer Export Format
PT J
TI Dual symbiosis in a Bathymodiolus sp mussel from a methane seep on the gabon continental margin (southeast Atlantic): 16S rRNA phylogeny and distribution of the symbionts in gills
BT 
AF DUPERRON, Sebastien
   NADALIG, Thierry
   CAPRAIS, Jean-Claude
   SIBUET, Myriam
   FIALA MEDIONI, Aline
   AMANN, Rudolf
   DUBILIER, Nicole
AS 1:1,2;2:2;3:2;4:2;5:3;6:1;7:1;
FF 1:PDG-DOP-DCB-EEP-LEP;2:PDG-DOP-DCB-EEP-LEP;3:PDG-DOP-DCB-EEP-LEP;4:PDG-CST;5:;6:;7:;
C1 Max Planck Inst Marine Microbiol, D-28359 Bremen, Germany.
   IFREMER, Ctr Brest, Dept Environm Profond, Plouzane, France.
   Univ Paris 06, Banyuls sur Mer, France.
C2 INST MAX PLANCK (MARINE MICROBIOL), GERMANY
   IFREMER, FRANCE
   UNIV PARIS 06, FRANCE
SI BREST
SE PDG-DOP-DCB-EEP-LEP
   PDG-CST
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
IF 3.818
TC 127
UR https://archimer.ifremer.fr/doc/2005/publication-1266.pdf
LA English
DT Article
CR BIOZAIRE 2
   ZAIANGO-BIOL2
BO Anchored Buoy
   Thalassa
DE ;Gabon;Southeast Atlantic;Methane seep;Hydrothermal vent;Deep sea;rRNA analysis;Methane concentration;Gill epithelium;Symbiosis;Bacteria;Mytilidae
AB Deep-sea mussels of the genus Bathymodiolus (Bivalvia: Mytilidae) harbor symbiotic bacteria in their gills and are among the dominant invertebrate species at cold seeps and hydrothermal vents. An undescribed Bathymodiolus species was collected at a depth of 3,150 m in a newly discovered cold seep area on the southeast Atlantic margin, close to the Zaire channel. Transmission electron microscopy, comparative 16S rRNA analysis, and fluorescence in situ hybridization indicated that this Bathymodiolus sp. lives in a dual symbiosis with sulfide- and methane-oxidizing bacteria. A distinct distribution pattern of the symbiotic bacteria in the gill epithelium was observed, with the thiotrophic symbiont dominating the apical region and the methanotrophic symbiont more abundant in the basal region of the bacteriocytes. No variations in this distribution pattern or in the relative abundances of the two symbionts were observed in mussels collected from three different mussel beds with methane concentrations ranging from 0.7 to 33.7 mu M. The 16S rRNA sequence of the methanotrophic symbiont is most closely related to those of known methanotrophic symbionts from other bathymodiolid mussels. Surprisingly, the thiotrophic Bathymodiolus sp. 16S rRNA sequence does not fall into the monophyletic group of sequences from thiotrophic symbionts of all other Bathymodiolus hosts. While these mussel species all come from vents, this study describes the first thiotrophic sequence from a seep mussel and shows that it is most closely related (99% sequence identity) to an environmental clone sequence obtained from a hydrothermal plume near Japan.
PY 2005
PD APR
SO Applied and environmental microbiology
SN 0099-2240
PU American society for microbiology
VL 71
IS 4
UT 000228338000003
BP 1694
EP 1700
DI 10.1128/AEM.71.4.1694-1700.2005
ID 1266
ER

EF