FN Archimer Export Format
PT J
TI Detection of cytotoxin-hemolysin mRNA in nonculturable populations of environmental and clinical Vibrio vulnificus strains in artificial seawater
BT 
AF FISCHER LE SAUX, Marion
   HERVIO HEATH, Dominique
   LOAEC, Solen
   COLWELL, Rita
   POMMEPUY, Monique
AS 1:;2:;3:;4:;5:;
FF 1:;2:PDG-DEL-MP-MIC;3:PDG-DEL-MP-MIC;4:;5:PDG-DEL-MP-MIC;
C1 IFREMER, DEL MP, Microbiol Lab, F-29280 Plouzane, France.
   Univ Maryland, Inst Biotechnol, Ctr Marine Biotechnol, Baltimore, MD USA.
C2 IFREMER, FRANCE
   UNIV MARYLAND, USA
SI BREST
SE PDG-DEL-MP-MIC
IN WOS Ifremer jusqu'en 2018
   copubli-int-hors-europe
IF 3.691
TC 49
UR https://archimer.ifremer.fr/doc/2002/publication-1269.pdf
LA English
DT Article
DE ;Artificial seawater;Reverse transcription PCR;Cytotoxin Hemolysin;Molecular detection method;Vibrio vulnificus
AB The objective of this study was to develop a molecular detection method that better estimates the potential risk associated with the presence of Vibrio vulnificus. For that purpose, we applied seminested reverse transcription-PCR (RT-PCR) to viable but nonculturable (VBNC) populations of V. vulnificus and targeted the cytotoxin-hemolysin virulence gene vvhA. Three strains, two environmental, IF Vv10 and IF Vv18, and one clinical, C7184, were used in this study. Artificial seawater, inoculated with mid-log-phase cells, was maintained at 4degreesC. VBNC cells resulted after 3, 6, and 14 days for C7184, IF Vv18, and IF Vv10, respectively. Our data indicate that seminested RT-PCR is sensitive for the detection of vvhA mRNA in artificial seawater when exclusively nonculturable bacteria are present. This is the first report of the expression of a toxin gene in VBNC V. vulnificus. Moreover, vvhA transcripts were shown to persist in nonculturable populations over a 4.5-month period, with a progressive decline of the signal over time. This result indicates that special attention should be given to the presence of potentially pathogenic VBNC cells in environmental samples when assessing public health risk.
PY 2002
PD NOV
SO Applied and environmental microbiology
SN 0099-2240
PU American society for microbiology
VL 68
IS 11
UT 000179027600055
BP 5641
EP 5646
DI 10.1128/AEM.68.11.5641-5646.2002
ID 1269
ER

EF