FN Archimer Export Format PT J TI Oligonucleotide quartz crystal microbalance sensor for the microalgae Alexandrium minutum (Dinophyceae) BT AF LAZERGES, M PERROT, H ANTOINE, Elisabeth DEFONTAINE, Audrey COMPERE, Chantal AS 1:1;2:1;3:2;4:2;5:3; FF 1:;2:;3:PDG-DOP-DCN-EMP-PHYC;4:PDG-DOP-DCN-EMP-PN;5:PDG-DOP-DCB-ERT-IC; C1 Univ Paris 06, CNRS, LISE, F-75252 Paris 05, France. IFREMER, Ctr Nantes, F-44311 Nantes, France. IFREMER, Ctr Brest, F-29280 Plouzane, France. C2 UNIV PARIS 06, FRANCE IFREMER, FRANCE IFREMER, FRANCE SI NANTES BREST SE PDG-DOP-DCN-EMP-PHYC PDG-DOP-DCN-EMP-PN PDG-DOP-DCB-ERT-IC IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 4.132 TC 23 UR https://archimer.ifremer.fr/doc/2006/publication-1354.pdf LA English DT Article DE ;DNA;Alexandrium minutum;Microbalance AB We report the immobilization on a gold surface of a 20-base DNA probe labeled with disulfide group and on the selective hybridization with the complementary 20-base DNA strand. The oligonucleotide probe is the complementary strand of a partial sequence of the gene encoding for a large ribosomal RNA sub-unit which is a coding sequence of Alexandrium minutum DNA, a microalgae that produces neurotoxins responsible for paralytic shellfish poisoning on European and Asian coasts. The kinetics of DNA probe immobilization and hybridization were monitored in situ by using a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the setup is stable to within a few hertz, corresponding to the nanogram, scale, for 3 h and makes it possible to follow frequency change from immobilization of the probe to hybridization of the complementary DNA target. This setup constitutes a biosensor, which is sensitive and selective, and the hybridization ratio between hybridized complementary DNA and immobilized DNA probes is 47%. PY 2006 SO Biosensors and Bioelectronics SN 0956-5663 PU Elsevier VL 21 IS 7 UT 000234644400046 BP 1355 EP 1358 DI 10.1016/j.bios.2005.05.013 ID 1354 ER EF