FN Archimer Export Format PT C TI Isolement et caracterisation d'une proteine membranaire ovocytaire capable de fixer specifiquement la vitellogenine OT Isolation and properties of ovocyte-membrane proteins able to fix vitellogenin specifically BT AF JUGAN, P VANHERP, F AS 1:;2:; FF 1:;2:; UR https://archimer.ifremer.fr/doc/1987/acte-1382.pdf LA French DT Proceedings paper DE ;Orconectes limosus;Malacostraca;Oogenesis;Biochemical analysis;Cell membranes AB After solubilization of ovocyte-membranes from the crayfish Orconectes limosus , a component with a molecular weight of 30 kDa and capable to fix vitellogenin specifically, could be characterized by SDS-PAGE and by electroblotting. Because this isolated component is sensible to the digestion of proteolytic enzymes such as pronase, it is assumed that it is a protein. A polyclonal antiserum is produced against this component and its tissue-specificity is verified by ELISA. PY 1987 PD JUL CT Actes de colloques. Ifremer. Brest [ACTES COLLOQ. IFREMER.]. 1988 ID 1382 ER EF