FN Archimer Export Format
PT J
TI Purification and characterization of infectious myonecrosis virus of penaeid shrimp
BT 
AF POULOS, Bonnie
   TANG, K
   PANTOJA, C
   BONAMI, Jean-Robert
   LIGHTNER, D
AS 1:1;2:1;3:1;4:2,3;5:1;
FF 1:;2:;3:;4:;5:;
C1 Univ Arizona, Aquaculture Pathol Lab, Dept Vet Sci & Microbiol, Tucson, AZ 85721 USA.
   CNRS, IFREMER, UM2, UMR 5098, F-34095 Montpellier 5, France.
C2 UNIV ARIZONA, USA
   CNRS, FRANCE
   IFREMER, FRANCE
SI MONTPELLIER
SE PDG-DOP-DCN-AGSAE-GPIA
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-int-hors-europe
IF 3.11
TC 129
UR https://archimer.ifremer.fr/doc/2006/publication-1504.pdf
LA English
DT Article
DE ;Purification;Myonecrosis;Virus;Shrimp;Penaeid
AB The causative agent of myonecrosis affecting cultured Penaeus vannamei in Brazil was demonstrated to be a virus after purification of the agent from infected shrimp tissues. Purified viral particles were injected into specific pathogen-free P. vannamei, resulting in a disease that displayed the same characteristics as those found in the original shrimp used for purification. The virus was named infectious myonecrosis virus (IMNV). The viral particles were icosahedral in shape and 40 nm in diameter, with a buoyant density of 1(.)366 g ml(-1) in caesium chloride. The genome consisted of a single, double-stranded (dsRNA) molecule of 7560 bp. Sequencing of the viral genome revealed two non-overlapping open reading frames (ORFs). The 5' ORF (ORF 1, nt 136-4953) encoded a putative RNA-binding protein and a capsid protein. The coding region of the RNA-binding protein was located in the first half of ORF 1 and contained a dsRNA-binding motif in the first 60 aa. The second half of ORF 1 encoded a capsid protein, as determined by amino acid sequencing, with a molecular mass of 106 kDa. The 3' ORF (ORF 2, nt 5241-7451) encoded a putative RNA-dependent RNA polymerase (RdRp) with motifs characteristic of totiviruses. Phylogenetic analysis based on the RdRp clustered IMNV with Giardia lamblia virus, a member of the family Totiviridae. Based on these findings, IMNV may be unique member of the Totiviridae or may represent a new dsRNA virus family that infects invertebrate hosts.
PY 2006
PD APR
SO JOURNAL-OF-GENERAL-VIROLOGY
SN 0022-1317
PU Journal of General Virology
VL 87
UT 000236446400029
BP 987
EP 996
DI 10.1099/vir.0.81127-0
ID 1504
ER

EF