Norwalk virus-specific binding to oyster digestive tissues

Type Article
Date 2006-06
Language English
Author(s) Le Guyader Soizick1, Loisy Fabienne1, Atmar R2, Hutson A2, Estes M2, Ruvoen-Clouet N3, 4, 5, Pommepuy Monique1, Le Pendu J4, 5
Affiliation(s) 1 : IFREMER, F-44311 Nantes 3, France.
2 : Baylor Coll Med, Houston, TX 77030 USA.
3 : Ecole Natl Vet, Nantes, France.
4 : Inst Natl Sante & Rech Med, Nantes, France.
5 : Univ Nantes, F-44035 Nantes, France.
Source Emerging infectious diseases (1080-6040) (CDC), 2006-06 , Vol. 12 , N. 6 , P. 931-936
WOS© Times Cited 167
Keyword(s) Bioaccumulation, Shellfish, Escherichia coli, Pathogen, Oyster digestive tissue, Noroviruses
Abstract The primary pathogens related to shellfishborne gastroenteritis outbreaks are noroviruses. These viruses show persistence in oysters, which suggests an active mechanism of virus concentration. We investigated whether Norwalk virus or viruslike particles bind specifically to oyster tissues after bioaccumulation or addition to tissue sections. Since noroviruses attach to carbohydrates of the histo-blood group family, tests using immunohistochemical analysis were performed to evaluate specific binding of virus or viruslike particles to oyster tissues through these ligands. Viral particles bind specifically to digestive ducts (midgut, main and secondary ducts, and tubules) by carbohydrate structures with a terminal N-acetylgalactosamine residue in an alpha linkage (same binding site used for recognition of human histo-blood group antigens). These data show that the oyster can selectively concentrate a human pathogen and that conventional depuration will not eliminate noroviruses from oyster tissue.
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Le Guyader Soizick, Loisy Fabienne, Atmar R, Hutson A, Estes M, Ruvoen-Clouet N, Pommepuy Monique, Le Pendu J (2006). Norwalk virus-specific binding to oyster digestive tissues. Emerging infectious diseases, 12(6), 931-936. Open Access version : https://archimer.ifremer.fr/doc/00000/1542/