TY - JOUR T1 - Norwalk virus-specific binding to oyster digestive tissues A1 - Le Guyader,Soizick A1 - Loisy,Fabienne A1 - Atmar,R A1 - Hutson,A A1 - Estes,M A1 - Ruvoen-Clouet,N A1 - Pommepuy,Monique A1 - Le Pendu,J AD - IFREMER, F-44311 Nantes 3, France. AD - Baylor Coll Med, Houston, TX 77030 USA. AD - Ecole Natl Vet, Nantes, France. AD - Inst Natl Sante & Rech Med, Nantes, France. AD - Univ Nantes, F-44035 Nantes, France. UR - https://archimer.ifremer.fr/doc/00000/1542/ KW - Bioaccumulation KW - Shellfish KW - Escherichia coli KW - Pathogen KW - Oyster digestive tissue KW - Noroviruses N2 - The primary pathogens related to shellfishborne gastroenteritis outbreaks are noroviruses. These viruses show persistence in oysters, which suggests an active mechanism of virus concentration. We investigated whether Norwalk virus or viruslike particles bind specifically to oyster tissues after bioaccumulation or addition to tissue sections. Since noroviruses attach to carbohydrates of the histo-blood group family, tests using immunohistochemical analysis were performed to evaluate specific binding of virus or viruslike particles to oyster tissues through these ligands. Viral particles bind specifically to digestive ducts (midgut, main and secondary ducts, and tubules) by carbohydrate structures with a terminal N-acetylgalactosamine residue in an alpha linkage (same binding site used for recognition of human histo-blood group antigens). These data show that the oyster can selectively concentrate a human pathogen and that conventional depuration will not eliminate noroviruses from oyster tissue. Y1 - 2006/06 PB - CDC JF - Emerging infectious diseases SN - 1080-6040 VL - 12 IS - 6 SP - 931 EP - 936 ID - 1542 ER -