FN Archimer Export Format PT J TI Impact of food availability on energy storage and defense related hemocyte parameters of the Pacific oyster Crassostrea gigas during an experimental reproductive cycle BT AF DELAPORTE, Maryse SOUDANT, P LAMBERT, C MOAL, Jeanne POUVREAU, Stephane SAMAIN, Jean-Francois AS 1:2;2:1;3:1;4:2;5:2;6:2; FF 1:PDG-DOP-DCB-PFOM-PI;2:;3:;4:PDG-DOP-DCB-PFOM-PI;5:PDG-DOP-DCB-PFOM-PI;6:PDG-DOP-DCB-PFOM-PI; C1 Univ Bretagne Occidentale, Inst Univ Europeen Mar, Lab Sci Environm Marin, UMR 6539, F-29280 Plouzane, France. IFREMER, Ctr Brest, Lab Physiol Invertebres, F-29280 Plouzane, France. C2 UBO, FRANCE IFREMER, FRANCE SI BREST ARGENTON SE PDG-DOP-DCB-PFOM-PI IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 2.081 TC 94 UR https://archimer.ifremer.fr/doc/2006/publication-1549.pdf LA English DT Article DE ;Reactive oxygen species production;Reproduction;Phagocytosis;Hemocyte parameters;Energy storage;Crassostrea gigas AB The aim of this study was to test the effect of food quantity on energy storage and defense capacities of oysters during a reproductive cycle. One-year-old Crassostrea gigas oysters were fed two different dietary rations (4% and 12% of oyster dry weight in algal dry weight per day) in controlled experimental conditions over an annual cycle. Oyster dry weights, carbohydrate and lipid contents, energetic adenylate charge, and hemocyte parameters of oysters were significantly affected by reproductive processes related to seasonal temperature variation and, to a lesser extent, by the dietary rations. Energy parameters decreased during gametogenesis as gonads developed then increased during the gonad resorption phase. The additional energy provided to oysters fed the 12% diet compared to oysters fed the 4% diet was allocated mainly to the development of more gonad tissue. Regardless of diet, hemocyte concentrations were also seasonally affected. Hemocyte concentrations were low during gametogenesis and significantly increased during the gonadal resorption and tissue restoration phase. Phagocytic activity and adhesive capacity of hemocytes were temporarily inhibited during gametogenesis and were at their lowest levels in June. Oysters fed the 12% diet had significantly higher hemocyte concentrations and lower phagocytosis activity and reactive oxygen species production compared to those fed the 4% diet. PY 2006 PD APR SO Aquaculture SN 0044-8486 PU Elsevier VL 254 IS 1-4 UT 000237838800060 BP 571 EP 582 DI 10.1016/j.aquaculture.2005.10.006 ID 1549 ER EF