Development of genomic probes for detection of hepatitis A virus and enteroviruses in shellfish

Other titles Mise au point de sondes génomiques pour la recherche du virus de l'hépatite A et des entérovirus dans les coquillages
Type Proceedings paper
Date 1992-04
Language English
Author(s) Apaire M, Leguyader F, Besse B, Billaudel S
Meeting 2. Conference Internationale sur la Purification des Coquillages, Rennes (France), 6-8 Apr 1992
Source Actes de colloques. Ifremer. Brest [ACTES COLLOQ. IFREMER.]. 1995
Keyword(s) Mollusca, Enterovirus, Hepatitis A virus, Picornaviridae, RNA probes, DNA probes, Seafood, Biology, Sensors, DNA, RNA, Viruses, Detection, Marine molluscs, Shellfish
Abstract The development of molecular biology techniques has modified research procedures for certain viruses, particularly hepatitis A virus (HAV) and enteroviruses. Accordingly, we designed cDNA and RNA genomic probes to detect viruses in shellfish in the environment. Better sensitivity was noted with RNA than cDNA probes: only 17/83 shellfish samples were positive with HAV and enterovirus DNA probes, where as 42/83 were positive with HAV and enterovirus RNA probes.
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Apaire M, Leguyader F, Besse B, Billaudel S (1992). Development of genomic probes for detection of hepatitis A virus and enteroviruses in shellfish. Actes de colloques. Ifremer. Brest [ACTES COLLOQ. IFREMER.]. 1995. https://archimer.ifremer.fr/doc/00000/1613/