||Évaluation par biologie moléculaire de la contamination par entérovirus et virus de l'hépatite A des coquillages de l'estuaire de la Loire
||Leguyader F, Apaire M, Besse B, Billaudel S
||2. Conference Internationale sur la Purification des Coquillages, Rennes (France), 6-8 Apr 1992
||Actes de colloques. Ifremer. Brest [ACTES COLLOQ. IFREMER.]. 1995
||France, Loire R., ANE, France, Pays de Loire, Loire Estuary, Hepatitis A virus, Enterovirus, Bivalvia, Hybridization analysis, Seafood, Estuaries, Rivers, Sensors, RNA, Disease detection, Viruses, Shellfish, Biology
||Riboprobes developed in our laboratory were used for detection of enteroviruses and hepatitis A virus (HAV) in shellfish (cockles and mussels). After concentration elution, virus nucleic acids were extracted and purified. Hybridization was performed on a solid support according to the dot-blot technique, with (32P) UTP labelling of probes during transcription. Results show high shellfish contamination in samples: 63% enteroviruses and 67% HAV. Cockles were particularly contaminated: 89% enteroviruses and 84% HAV.