| Other titles |
Évaluation par biologie moléculaire de la contamination par entérovirus et virus de l'hépatite A des coquillages de l'estuaire de la Loire |
| Type |
Proceedings paper |
| Date |
1992-04 |
| Language |
English |
| Author(s) |
Leguyader F, Apaire M, Besse B, Billaudel S |
| Meeting |
2. Conference Internationale sur la Purification des Coquillages, Rennes (France), 6-8 Apr 1992 |
| Source |
Actes de colloques. Ifremer. Brest [ACTES COLLOQ. IFREMER.]. 1995 |
| Keyword(s) |
France, Loire R., ANE, France, Pays de Loire, Loire Estuary, Hepatitis A virus, Enterovirus, Bivalvia, Hybridization analysis, Seafood, Estuaries, Rivers, Sensors, RNA, Disease detection, Viruses, Shellfish, Biology |
| Abstract |
Riboprobes developed in our laboratory were used for detection of enteroviruses and hepatitis A virus (HAV) in shellfish (cockles and mussels). After concentration elution, virus nucleic acids were extracted and purified. Hybridization was performed on a solid support according to the dot-blot technique, with (32P) UTP labelling of probes during transcription. Results show high shellfish contamination in samples: 63% enteroviruses and 67% HAV. Cockles were particularly contaminated: 89% enteroviruses and 84% HAV. |
| Full Text |
| File |
Pages |
Size |
Access |
| acte-1616.pdf |
10 |
1 MB |
Open access |
|
