FN Archimer Export Format PT J TI Molecular identification of Vibrio tapetis, the causative agent of the brown ring disease of Ruditapes philippinarum BT AF PAILLARD, Christine GAUSSON, Sabrina NICOLAS, Jean-Louis LE PENNEC, Jean Paul HARAS, Dominique AS 1:1;2:1;3:3;4:2;5:2; FF 1:;2:;3:PDG-DOP-DCB-PFOM-PI;4:;5:; C1 Univ Bretagne Occidentale, Inst Univ Europeen Mer, Lab Sci Environm Marin, UMR 6539, F-29280 Plouzane, France. Univ Bretagne Sud, Lab Biotechnol & Chim Marines, EA 3884, F-56321 Lorient, France. IFREMER, Ctr Brest, DRV RA PI, Lab Physiol Invertebres, F-29280 Plouzane, France. C2 UBO, FRANCE UBS, FRANCE IFREMER, FRANCE SI BREST SE IUEM PDG-DOP-DCB-PFOM-PI IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 2.081 TC 30 UR https://archimer.ifremer.fr/doc/2006/publication-1692.pdf LA English DT Article DE ;DOT BLOT hybridisation;SSP PCR;Vibriosis;Bivalve;Detection;Diagnostic AB Vibrio tapetis is the marine bacterium responsible for the brown ring disease (BRD) affecting die manila clam, Ruditapes philippinarum. Identification of V tapelis has been previously performed using biochemical criteria and serological procedures. All of these methods are time consuming and ill-adapted to individual screening. This study describes an oligonucleotidic probe (Vt446) and two PCR primers, deduced from the 16S rDNA sequence, allowing a fast and specific V tapetis identification using dot blot hybridisation and species-specific primed PCR (SSP-PCR). The probe and primers have been tested on 60 strains, including referenced Vibrio sp., Gram negative and positive bacteria, marine bacteria samples and isolated clam bacteria. For all the 19 V tapetis strains, the results of PCR assays consistently corroborated those of the agglutination tests. The detection limit was estimated to be 10(2) CFU ml(-1). The SSP-PCR method has resulted in V tapetis detection in larvae, m diseased clams, and in asymptomatic broodstock clams that later developed BRD. In conclusion, the two SSP-PCR primers were useful for direct and fast identification of V tapetis strains isolated in clams, and are well suited for the screening of individual R. philippilnarum broodstock clams and larvae from the hatchery. (C) 2005 Elsevier B.V. All rights reserved. PY 2006 PD MAR SO Aquaculture SN 0044-8486 PU Elsevier VL 253 IS 1-4 UT 000236898700004 BP 25 EP 38 DI 10.1016/j.aquaculture.2005.03.047 ID 1692 ER EF