FN Archimer Export Format
PT J
TI Pollutant effects on Pacific oyster, Crassostrea gigas (Thunberg), hemocytes: Screening of 23 molecules using flow cytometry
BT 
AF GAGNAIRE, Beatrice
   THOMAS GUYON, H
   BURGEOT, Thierry
   RENAULT, Tristan
AS 1:1;2:2;3:3;4:1;
FF 1:PDG-DOP-DCN-AGSAE-LGP;2:;3:PDG-DOP-DCN-BE-LBEX;4:PDG-DOP-DCN-AGSAE-LGP;
C1 IFREMER, LPG, F-17390 La Tremblade, France.
   Univ La Rochelle, LBEM, FRE 2727, La Rochelle, France.
   IFREMER, Dept Polluants Chim, Nantes, France.
C2 IFREMER, FRANCE
   UNIV LA ROCHELLE, FRANCE
   IFREMER, FRANCE
SI LA TREMBLADE
   NANTES
SE PDG-DOP-DCN-AGSAE-LGP
   PDG-DOP-DCN-BE-LBEX
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 1.4
TC 87
UR https://archimer.ifremer.fr/doc/2006/publication-1695.pdf
LA English
DT Article
DE ;Toxicity;Pesticides;PCBs;PAHs;Crassostrea gigas;Pacific oyster;Hemocytes;Flow cytometry;Cellular activities
AB The shellfish industry is an important economic activity in France, occurring mostly in estuarine zones subject to pollution due to anthropogenic activities. The harmful effects of pollutants on species inhabiting these estuarine zones are not well known. Among marine species, bivalve mollusks---particularly Pacific oyster, Crassostrea gigas-may serve a model of interest. The species is sedentary and filter-feeding, which favors bioaccumulation of pollutants in their tissues. Oysters may be suitable for studies on disturbance by pollutants of physiological activities, among which defense mechanisms are poorly documented in bivalves. In this study, effects of pollutants on hemocyte functions were monitored in Pacific oyster, C. gigas. Hemocytes were exposed in vitro to selected pollutants. The strategy for investigating the effects of pollutants on hemocyte functions is based on several biomarkers, which is more relevant than that of published papers based on single-endpoint experiments. Pollutants belonging to the most important groups of xenobiotics (PAHs, PCBs, and pesticides) were selected and their effect on hemocyte activities was analyzed using flow cytometry. Twenty-three pollutants were tested and eight of them showed significant modulation of hemocyte activities. PAHs and PCB 77 induced a decrease of hemocyte activity after an incubation periods of 4 and 24 h at 200 mu mol/L. Three pesticides (2,4D, paraoxon, and chlorothalonil) modulated hemocyte activities. A mixture of eight pesticides also decreased phagocytotic activity. This study is one of the first to investigate the effects of so many pollutants on hemocyte functions at the same time and therefore allows a real comparison of different pollutant effects.
PY 2006
SO Cell Biology and Toxicology
SN 0742-2091
PU Kluwer
VL 22
IS 1
UT 000235139300001
BP 1
EP 14
DI 10.1007/s10565-006-0011-6
ID 1695
ER

EF